Summary
A simplified single radial immunodiffusion (SRID) method to detect HBsAg particles albumin receptor ac tivity in regard to polymerized human serum albumin (pHSA) (binding-activity of pHSA=pHSA-BA or albumin receptor activity) was developed and its significance in HBeAg-positive hepatitis B patients after a one year follow-up study was discussed. The pHSA-BA determined by the simplified SRID method was clas sified into 5 grades; +++, ++, +, ±and -, and the results of this grading of test sera agreed well with those ob tained by the ELISA quantitative assay. According to the results of one year follow-up study in sero-changes of HBeAg in 35 HBeAg-positive cases, 7 cases with (+++) pHSA-BA did not show HBeAg sero-change, but of 16 cases with (++) pHSA-BA and 12 cases with (+) pHSA-BA 3 (18.8%) and 7 (58.3%) cases showed HBeAg sero changes, respectively. These results suggest that the present simplified SRID detection of albumin receptor on HBsAg particles might be a useful method for the laboratory investigation of hepatitis B patients.
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This work was supported by a Grant-in Aid (No. 58570321) for Scientific Research from the Ministry of Education, Science and Culture of Japan in 1983.
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Tsuji, T., Takahashi, K., Naito, K. et al. Simplified single radial immunodiffusion detection of albumin receptors on HBsAG particles and its significance regarding sero-changes of hbeag in hepatitis-B patients. Gastroenterol Jpn 19, 116–120 (1984). https://doi.org/10.1007/BF02806933
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DOI: https://doi.org/10.1007/BF02806933