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Increased expression of Epstein-Barr virus in primary biliary cirrhosis patients

  • Liver, Pancreas and Biliary Tract
  • Published:
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Summary

Peripheral blood mononuclear cells (PBMC, n = 26), formalin-fixed paraffin-embedded liver tissues (n = 11) and saliva (n = 15) of primary biliary cirrhosis (PBC) patients were used for the detection of Epstein-Barr virus (EBV) sequences by polymerase chain reaction (PCR) assay. The semiquantitative analysis of EBV-DNA was also carried out in a reconstructive experiment using an EBV-infected cell line. The PBMCs of PBC patients showed increased levels of EBV-DNA (61%) in contrast to chronic active hepatitis patients (19%), liver cirrhosis patients (14%) and healthy individuals (11%). Furthermore, formalinfixed paraffin-embedded liver tissues, as well as saliva from PBC patients, also demonstrated increased levels of EBV-DNA when compared to healthy individuals and those with other liver diseases. The increased levels of EBV-DNA in the PBMC, liver tissue and saliva of the PBC patients suggest that those patients may have a depressed immune function against EBV infection.

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Authors and Affiliations

  1. Third Department of Internal Medicine, Kagawa Medical School, 1750-1 Ikenobe, Miki-cho, Kita-gun, 761-07, Kagawa, Japan

    Syed Ahmed Morshed & Mikio Nishioka M.D. (Professor of Internal Medicine)

  2. Medical Research Institute, Department of Virology and Immunology, Tokyo Medical and Dental University, Tokyo, Japan

    Ichiro Saito

  3. Department of Pathology, Nihon University School of Dentistry, Tokyo, Japan

    Kazuo Komiyama & Itaru Moro

Authors
  1. Syed Ahmed Morshed
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  2. Mikio Nishioka M.D.
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  3. Ichiro Saito
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  4. Kazuo Komiyama
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  5. Itaru Moro
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Morshed, S.A., Nishioka, M., Saito, I. et al. Increased expression of Epstein-Barr virus in primary biliary cirrhosis patients. Gastroenterol Jpn 27, 751–758 (1992). https://doi.org/10.1007/BF02806528

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  • DOI: https://doi.org/10.1007/BF02806528

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