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Hypersecretion of biliary fatty acids in patients with exocrine pancreatic disease

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Summary

The fatty acid composition of bile secreted into the duodenum in the first 10 min after an intravenous (i.v.) injection of Boots secretin (2 CHRu kg-1) has been analysed by gas liquid chromatography in 11 healthy volunteers, 8 patients without pancreatic disease, 27 patients with exocrine pancreatic disease who had not altered their diet substantially (acute pancreatitis 8; chronic pancreatitis 16; cancer 3) and 11 patients with exocrine pancreatic disease on low fat intakes (40 g/day) for at least 6 months. The mean values for total fatty acid outputs (after back transformation of the logged data) were significantly higher in each subgroup of patients with pancreatic disease on their usual diets (acute 134, chronic 189, cancer 235 mg) than in the two subgroups of controls (30 and 55 mg), due to significant increases in the outputs of every fatty acid, C16:0 through to C22:5. This finding, which was usually not apparent in patients with pancreatic disease on low-fat diets, may reflect the combined influence of dietary fat intakes and hepatic enzyme induction. Comparison of the fatty acid outputs in endoscopically collected bile and duodenal juice after separate injections of secretin three hours apart indicate that: (a) analysis of duodenal juice within 10 min of stimulation by Boots secretin provides valuable information on the composition of hepatic bile; (b) the increased phospholipid output in the untreated patients is due to hypersecretion and does not merely represent a ‘washout’ phenomenon.

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Abbreviations

C16:0:

Palmitic acid

C16:1:

palmitoleic acid

C17:0:

margaric acid

C18:0:

stearic acid

C18:1c:

oleic acid

C18:

lt, eliadic acid

C18:2:

linoleic acid

C20:0:

arachidic acid

C20:4:

arachidonic acid

C22:5:

clupanodonic acid

References

  1. Blomstrand, R. and Ekdahl, P., Fatty acid pattern of human bile under normal and pathological conditions, Proc. Soc. Exp. Biol. Med., 1960; 104: 205–209.

    PubMed  CAS  Google Scholar 

  2. Braganza, J.M., Wickens, D.G., Cawood, P. and Dormandy, T.L., Lipid peroxidation (free radicaloxidation) products in bile from patients with pancreatic disease, Lancet, 1983; i; 375–379.

    Article  Google Scholar 

  3. Braganza, J.M., Hunt, L.P. and Warwick, F., Relationship between pancreatic exocrine function and ductal morphology in chronic pancreatitis, Gastroenterology, 1982; 82: 1341–1347.

    PubMed  CAS  Google Scholar 

  4. Burton, P., Evans, D.G., Harper, A.A., Howat, H.T., Oleesky, S., Scott, J.E. and Varley, H., A test of pancreatic function in man based on the analysis of duodenal contents after administration of secretin and pancreozymin, Gut, 1960; I: 111–124.

    Article  Google Scholar 

  5. Braganza, J.M., Howat, H.T. and Kay, G., The relative potency of the Crick-Harper-Raper unit and the GIH clinical unit of secretin, Gut, 1975; 16: 549–552.

    Article  PubMed  CAS  Google Scholar 

  6. Braganza, J.M., Herman, K., Hine, P., Kay, G. and Sandle, G.I., Pancreatic enzymes in human duodenal juice—a comparison of responses in secretin pancreozymin and Lundh-Borgstrom tests, Gut, 1978; 19: 358–366.

    Article  PubMed  CAS  Google Scholar 

  7. Burton, P., Harper, A.A., Howat, H.T., Scott, J.E. and Varley, H., The use of cholecystokinin to test gall bladder function in man, Gut, 1960; 1: 193–204.

    Article  PubMed  CAS  Google Scholar 

  8. Braganza, J.M., Kay, P., Kay, G. and Hunt, L.P., Increased bile pigment secretion in non-jaundiced patients with pancreatic disease, Mt. Sinai J. Med., 1986; 53:389–395.

    PubMed  CAS  Google Scholar 

  9. Warwick, F., Anderson, R.J.L. and Braganza, J.M., Sclerosing-cholangitis-like changes in pancreatic disease, Clin. Radiol., 1985; 36: 51–56.

    Article  PubMed  CAS  Google Scholar 

  10. Rose, P., Fraine, E., Hunt, L.P., Acheson, D.W.K. and Braganza, J.M., Dietary antioxidants and chronic pancreatitis, Hum. Nutr. Clin. Nutr., 1986; 40C: 151–164.

    Google Scholar 

  11. Reuben, A., Maton, P.N., Murphy, G.M. and Dowling, R.H., Bile lipid secretion in obese and nonobese individuals with and without gallstones, Clin. Sci., 1985; 69: 71–79.

    PubMed  CAS  Google Scholar 

  12. Soloway, R.D., Clark, M.L., Powell, K.M., Senior, J.R. and Brooks, F.P., Effects of secretin and bile salt infusions on canine bile composition and flow, Am. J. Physiol., 1972; 222: 681–683.

    PubMed  CAS  Google Scholar 

  13. Gordon, E.M., Douglas, M.C., Jabionski, P., Owen, J.A., Sali, A. and Watts, J.McK., Gastroduodenal hormones and bile-secretion studies in the isolated perfused pig liver, Surgery, 1972; 72: 708–721.

    PubMed  CAS  Google Scholar 

  14. Pissidis, A.G., Bombeck, C.T., Merchant, F. and Nyhus, L.M., Hormonal regulation of bile secretion: a study in the isolated perfused liver, Surgery, 19; 66: 1075–1084.

  15. Romanski, K.W. and Bochenek, W.J., Effect of secretin, pancreozymin OP-CCK, and glucagon on bile flow and bile lipid secretion in rats, Gut, 1983; 24: 803–806.

    Article  PubMed  CAS  Google Scholar 

  16. Braganza, J.M., Klass, H.J., Bell, M. and Sturniolo, G., Evidence of altered copper metabolism in patients with chronic pancreatitis, Clin. Sci., 1981; 60: 303–310.

    PubMed  CAS  Google Scholar 

  17. Worning, H., Mullertz, S., Thaysen, E.H. and Bang, H.O., pH and concentration of pancreatic enzymes in aspirates from the human duodenum during digestion of a standard meal in patients with biliary or hepatic disorders, Scand. J. of Gastroenterol, 1967; 2: 150–156.

    Article  CAS  Google Scholar 

  18. Klass, H.J., Sandle, G.I., Kay, P.M., Davies, P. and Braganza, J.M., Quantitation of tryptic responses to endogenous and exogenous stimulation in chronic pancreatitis, Digestion, 1986; in press.

  19. Balint, J.A., Beeler, D.A., Treble, D.H. and Spitzer, H.L., Studies on the biosynthesis of hepatic and biliary lecithins, Lipid Res., 1967; 8: 486–493.

    CAS  Google Scholar 

  20. Stein, O. and Stein, Y., Lecithin synthesis, intracellular transport, and secretion in rat liver, J. Cell. Biol., 1969; 40: 461–483.

    Article  PubMed  CAS  Google Scholar 

  21. Gregory, D.H., Vlahcevic, Z.R., Schatzki, P. and Swell, L., Mechanism of secretion of biliary lipids, J. Clin. Invest., 1975; 55: 105–114.

    Article  PubMed  CAS  Google Scholar 

  22. Wilgram, G.F. and Kennedy, E.P., Intracellular distribution of some enzymes catalyzing reactions in the biosynthesis of complex lipids, J. Biol. Chem., 1963; 238: 2165–2169.

    Google Scholar 

  23. Remmer, H. and Merker, H.J., Drug-induced changes in the liver endoplasmic reticulum: association with drug-metabolizing enzymes, Science, 1963; 142: 1657–1658.

    Article  PubMed  CAS  Google Scholar 

  24. Orrenius, S., Further studies on the induction of the drug-hydroxylating enzyme system of liver microsomes, J. Cell Biol., 1965; 26: 725–731.

    Article  CAS  PubMed  Google Scholar 

  25. Young, D.L., Powell, G. and McMillan, W.O., Phenobarbital-induced alterations in phosphatidylcholine and triglyceride synthesis in hepatic endoplasmic reticulum, J. Lipid Res. 1971; 12: 1–8.

    PubMed  CAS  Google Scholar 

  26. Robins, S.J. and Armstrong, M.J., Biliary lecithin secretion. 11. Effects of dietary choline on biliary lecithin synthesis, Gastroenterology, 1976; 70: 397–402.

    PubMed  CAS  Google Scholar 

  27. Redinger, R.N. and Small, D.M., The effect of phenobarbital upon bile salt synthesis and pool size, biliary lipid secretion and bile composition, J. Clin. Invest., 1973; 52: 161–172.

    Article  PubMed  CAS  Google Scholar 

  28. Soloway, R.D., Clark, M.L., Powell, K.M., Senior, J.R. and Brooks, F.P., Effects of secretin and bile salt infusions on canine bile composition and flow, Am. J. Physiol. 1972; 222: 681–683.

    PubMed  CAS  Google Scholar 

  29. Braganza, J.M., Altered clearance of sulphobromophthalein (BSP) in patients with pancreatic disease, Clin. Chim. Acta, 1984; 138: 163–173.

    Article  PubMed  CAS  Google Scholar 

  30. Acheson, D.W.K., Rose, P., Houston, J.B. and Braganza, J.M., Induction of cytochromes P450 in pancreatic disease: consequence, coincidence or cause? Clin. Chim. Acta, 1985; 153: 73–84.

    Article  PubMed  CAS  Google Scholar 

  31. Sandle, L.N., Acheson, D.W.K., Houston, J.B., Gowenlock, A.H. and Braganza, J.M., Increased urinary D-glucaric acid as an indicator of microsomal enzyme induction in pancreatic disease, Clin. Sci., 1985; 69 (suppl. 12): 10P.

    Google Scholar 

  32. Haboubi, N.J., Ali, H.H. and Braganza, J.M., Liver histology in patients with pancreatitis: a clue to aetiology? Mt. Sinai J. Med., 1986; in press.

  33. Durrington, P.N., Twentyman, O.P., Braganza, J.M. and Miller, J.P., Hypertriglyceridaemia and abnormalities of triglyceride catabolism persisting after pancreatitis. Int. J. Pancreatol., 1986; 1: 195–203.

    PubMed  CAS  Google Scholar 

  34. Rajmanickman, C., Satyanarayana Rao, M.R. and Padmanabhan, G., On the sequence of reactions leading to cytochrome P450 synthesis—effect of drugs, J. Biol. Chem., 1975; 250: 2305–2310.

    Google Scholar 

  35. Parker, G., Branigan, S., Houston, J.B. and Braganza, J.M., Potent induction of cytochromes P448 by corn oil in Syrian golden hamsters, Gut. 1986; abstract, in press.

  36. Braganza, J.M., Jolley, J.E. and Lee, W.R., Occupational volatile chemicals and pancreatitis: a link? Int. J. Pancreatol., 1986; 1: 1–19.

    Google Scholar 

  37. Wills, E.D., The role of the polyunsaturated fatty acid composition of the endoplasmic reticulum in the regulation of the fate of oxidative drug and carcinogen metabolism. In: M.J. Coon, A.H. Conney, R.W. Estabrook, H.V. Gelboin, J.R. Gillette and P.J. O’Brien (Eds.), Microsomes, Drug Oxidation and Chemical Carcinogenesis, Academic Press, London, 1980: 545–548.

    Google Scholar 

  38. Wade, A.E., Norred, W.P. and Evans, J.S., Lipids in drug detoxification. In: J.N. Hathcock and J. Coon (Eds.), Nutrition and Drug Interrelations, Academic Press, New York, 1978: 475–492.

    Google Scholar 

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Authors and Affiliations

  1. Department of Organic Chemistry, Manchester University, UK

    Mohammed Taj & George N. Smith

  2. Department of Dietetics, Royal Infirmary, M13 9WL, Manchester, UK

    Patricia Rose

  3. Department of Medical Computation, Royal Infirmary, M13 9WL, Manchester, UK

    Linda P. Hunt

  4. Department of Gastroenterology, Royal Infirmary, Oxford Road, M13 9WL, Manchester, UK

    Joan M. Braganza

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  1. Mohammed Taj
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  2. Patricia Rose
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  3. Linda P. Hunt
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  4. George N. Smith
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  5. Joan M. Braganza
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Taj, M., Rose, P., Hunt, L.P. et al. Hypersecretion of biliary fatty acids in patients with exocrine pancreatic disease. Int J Pancreatol 1, 309–326 (1986). https://doi.org/10.1007/BF02801864

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  • DOI: https://doi.org/10.1007/BF02801864

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