Summary
Attempts have been made to find out the biochemical changes peculiar to the gastric juice of the gastirc cancer patient, especially useful for the detection of early gastric cancer. For this purpose, high molecular substances, amino acids, enzymes and acid contained in gastric juice were examined by various methods. The results obtained by the physical examinations of high molecular substances showed that the electrophoretic or ultracentrifugal sedimentation analysis is incompetent as the diagnostic method of early gastric cancer, though some characteristic changes were found in advanced cancer. The increase of amino-nitrogen content or of histidine-arginine ratio was not so specific for early gastric cancer. The mucoprotein fraction of cancerous gastric juice contained large amount of TTC reducing substances. This method was not so useful for the detection of early cancer. The sialic acid, estimated by diphenylamine reagent, increased in mucoproteose fraction of cancerous gastric juice, but very slightly in early stage. Though the liver catalase depressing factor, cancer toxin or toxohormone, seemed to be a specific substance elaborated from cancer cells, the gastric juice of early gastric cancer does not contained it in a detectable amount by the mouse bioassay. Many enzymes were detected in gastric juice, and lactate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, β—glucuronidase, carbonic anhydrase and alkaline phosphatase increased in cancerous gastric juice. The elevation of these enzyme activities, however, was very slight in early stage. The isozyme pattern of pepsin may be useful for the differentiation of early gastric cancer. The continuous pH titration method has been designed. With this method, the cancer pattern was obtained even in almost cases of early gastric cancer. The continuous pH titration method, therefore, seemed to be of value in differentiating an early gastric cancer from benign gastric diseases.
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References
Sato, H.: Biologic Diagnosis of Cancer. J.Jap. Soc. Int. Med., 45: 563–600, 1956.
Sato, H.: The Biologic Diagnosis of Gastric Cancer. Proc. World Cong. Gastroenterology, Washington, D. C, U.S.A.: 991–999, 1958.
Sato, H., Yunoki, K., et al.: On the Biological Diagnosis of Gastric Cancer. Acta Med. Univ. Kagoshima., 1: 166–175, 1959.
Sato, H. and Yunoki, K.: Cancer Diagnosis by the Detection of Toxic Factor Peculiar to Cancer. Jap. J. Med., 2: 291–300, 1963.
Sato, H., Yunoki, K., et al.: Biochemical Changes Peculiar to the Gastric Juice of Gastric Cancer. Acta Med. Univ. Kagoshima., 7: 63–71, 1965.
Sato, H. and Yunoki, K.: Significance of High Molecular Substances in Gastric Juice for Diagnosis of the Gastric Diseases, Especially Gastric Carcinoma. Proc. 3rd World Cong. Gastroenterology, Recent Advances in Gastroenterology. I: 558–561, 1967.
Glass, G. B. and Boyd, L. J.: The Three Main Components of the Human Gastric Mucin ; Dissolved Mucoproteose, Dissolved Mucoprotein, and Mucoid of the Gastric Visible Mucus. I, II, III, Gastroenterology, 12 : 821, 835, 849, 1949.
Werner, I. and Odin, L.: On the Presence of Sialic Acid in Certain Glycoproteins and in Gangliosides. Acta Soc. Med. Upsalien. 57: 230, 1952.
Gottschalk, A.: The Chemistry and Biology of Sialic Acids and Related Substances. Cambridge University Press, Spottiswoode, Ballantyne and Co. Ltd., London and Colchester, 1960.
Nakahara, W. and Fukuoka, F.: Chemistry of Cancer Toxin, Toxohormone. American Lecture Series, Charles C Thomas Publisher, U. S. A., 1961.
Yunoki, K. and Griffin, A.C.: Purification of Toxohormone by Column Chromatography. Cancer Research, 20: 533–540, 1960.
Yunoki, K. and Griffin, A.C.: Composition and Properties of a Highly Purified Toxohormone Preparation. Cancer Research, 21: 537–544, 1961.
Yunoki, K.: On the Biologic Actions of Cancer Toxin. Proc. Jap. Soc. Clinical Biochemistry and Metabolism. I: 42–46, 1964.
Yunoki, K.: Enzyme Activities in Gastric Juice of the Patient with Gastric Diseases. Jap. J. Gastroenterology, 62 : 877–882, 1965.
Taylor, W.H.: Studies on Gastric Proteolysis, 1, 2., Biochem. J., 71: 73–83, 373–383, 1959.
Seijffers, M. J., Miller, L. L., and Segal, H.L.: The Separation of Pepsins from Human Gastric Juice, JAMA, 183: 998–1000, 1963.
Seijffers, M.J., Segal, H. L., and Miller, L.L.: Separation of Pepsin I, Pepsin II A, Pepsin IIB, and Pepsin III from Human Gastric Mucosa. Amer. J. Physiol., 205: 1099–1105, 1963.
Seijffers, M.J., Segal, H. L., and Miller, L.L.: Separation of pepsinogen I, Pepsinogen II, and Pepsinogen III from Human Gastric Mucosa. Amer. J. Physiol., 205: 1106–1112, 1963.
Segal, H.L., Miller, L. L., Turner, M. D., and Seijffers, M.J.: Chromatographie Separation of Human Pepsinogens and Pepsins. Proc. 3rd World Cong. Gastroenterology, Recent Advances in Gastroenterology, I: 418–421, 1967.
Busch, H.: An Introduction to the Biochemistry of the Cancer Cell. Academic Press, New York, London, 1962.
Busch, H.: Methods in Cancer Research, Vol. II; Academic Press, New York, London, 1967.
Piper, D.W., Macoun, M. L., Broderick, F.L., Fenton, B.H., and Builder, J.: The Diagnosis of Gastric Carcinoma by the Estimation of Enzyme Activity in Gastric Juice, Gastroenterology, 45: 614–620, 1963.
Bock, O. A. A. : The Concepts of Free Acid and Total Acid of the Gastric Juice, Lancet, 1101–1102, 1962.
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Yunoki, K. Secretion of gastric juice in early gastric cancer. Gastroenterol Jpn 2, 265–277 (1967). https://doi.org/10.1007/BF02801721
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DOI: https://doi.org/10.1007/BF02801721