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Leakage of immobilized IgG from therapeutic immunoadsorbents

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Abstract

In developing therapeutic immunoadsorbents (IAs), antibodies (IgG molecules) covalently immobilized on porous carriers, a leak of IgG was determined both in the storage test with buffers at 25 and 4°C and in contact with plasma at room temperature (RT). The amount of antibody released from therapeutic IAs must be minimized to avoid side effects during treatment. The amount of IgG released was

  1. a.

    Dependent on the amount of IgG immobilized

  2. b.

    Much greater with CNBr-activated Sepharose 4B, or FormylCellulofine as a support material than with aminopropyl CPG (controlled pore glass)

  3. c.

    Found to yield again during another storage in buffers after the IAs were washed and their buffers replaced with fresh ones and

  4. d.

    Decreased after the IAs were treated with glutaraldehyde (GA) solutions.

Whereas treating the IAs with GA solutions significantly reduced the amount of IgG released, it caused some deterioration of the adsorption characteristics of the IAs. An irradiation dose of 2.5 Mrad as a crosslinking procedure also reduced the amount of IgG released; its effect was comparable to that of 0.025% GA, the lowest concentration used.

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Authors and Affiliations

  1. Research and Development Division, Medical Supply and Equipment Department, Takeda Chemical Industries, Ltd., 17-85 Jusohonmachi 2-chome, 532, Yodogawa-ku, Osaka, Japan

    Hiroshi Sato, Teruhisa Kidaka & Masatake Hori

Authors
  1. Hiroshi Sato
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  2. Teruhisa Kidaka
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  3. Masatake Hori
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Sato, H., Kidaka, T. & Hori, M. Leakage of immobilized IgG from therapeutic immunoadsorbents. Appl Biochem Biotechnol 15, 145–158 (1987). https://doi.org/10.1007/BF02801315

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  • DOI: https://doi.org/10.1007/BF02801315

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