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Kinetically Controlled Enzyme-Catalyzed Synthesis of Kyotorphin

An Optimization Study

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Abstract

Regarding the α-chymotrypsin-catalyzed synthesis of Z-TyrArg-NH2, the effect on the reaction yield of the following experimental factors is discussed: DMF/buffer proportions, reaction temperature, and donor/nucleophile ratios. The experimental design shows that relatively better yields are obtained by increasing the reaction temperature and lowering the cosolvent proportion while maintaining a slight excess of nucleophile. The ascensional line reaches an optimal response surface very flat voided of a well-defined maximum.

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Authors and Affiliations

  1. Laboratory of Peptides, Biological Organic Chemistry Department, Centro de Investigación y Desarrollo, CSIC, Jorge Girona Salgado18-26, 08034, Barcelona, Spain

    P. Clapés, G. Valencia, F. Reig & J. M. García Anton

  2. Chemical Engineering Department, Faculty of Chemistry, University of Barcelona, Diagonal 647, 08028, Barcelona, Spain

    J. Mata

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  1. P. Clapés
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  2. G. Valencia
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  3. F. Reig
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  4. J. M. García Anton
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  5. J. Mata
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Clapés, P., Valencia, G., Reig, F. et al. Kinetically Controlled Enzyme-Catalyzed Synthesis of Kyotorphin. Appl Biochem Biotechnol 15, 89–96 (1987). https://doi.org/10.1007/BF02801310

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  • DOI: https://doi.org/10.1007/BF02801310

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