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An improved clearing method for GUS assay inArabidopsis endosperm and seeds

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An Erratum to this article was published on 01 September 2002

Abstract

Precise cellular localization of the GUS stain is notoriously difficult inArabidopsis seeds. Here we report an improved protocol for the clearing of seeds after GUS staining. Incubation in ethanol-acetic acid (EtAc) and Hoyer’s medium allows reliable cellular localization of the GUS, even in seeds from late developmental stages. This method also leads to the staining of nucleoli in the endosperm and embryo, facilitating nuclear counts in endosperm development.

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Author notes

  1. Biljana Stangeland

    Present address: Department of Chemistry and Biotechnology, Agricultural University of Norway, PO Box 5040, Aas

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    1. Biljana Stangeland
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    2. Zhian Salehian
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    Correspondence to Biljana Stangeland.

    Additional information

    An erratum to this article is available at http://dx.doi.org/10.1007/BF02782473.

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    Stangeland, B., Salehian, Z. An improved clearing method for GUS assay inArabidopsis endosperm and seeds. Plant Mol Biol Rep 20, 107–114 (2002). https://doi.org/10.1007/BF02799426

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    • DOI: https://doi.org/10.1007/BF02799426

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