Summary
Seven kinds of pancreatic ductal adenocarcinomas induced by N-nitrosobis(2-hydroxypropil) amine in Syrian golden hamsters were established as transplantable tumor lines on syngeneic animals. These tumor lines were all well or moderately differentiated adenocarcinomas, showing various velocities of growth, unrelated to the grade of histological differentiation. A cell line, designated HaP-Tl, was established in continuous tissue culture from one of these homografts. The cells grew in a monolayered sheet with an approximately 17-hour population doubling time. Chromosomal analysis revealed that the modal chromosomal number of the cell line was 44. HaP-Tl cells showed apparent tumorigenicity both on syngeneic hamsters and athymic nude mice, but they grew much faster when injected into the former animals. Morphological characteristics of HaP-Tl cells and tumors induced by HaP-Tl inoculation in both animals revealed apparent epithelial characteristics resembling ductal adenocarcinoma of the pancreas. These transplantable tumor models will contribute to the further investigation in the field of pancreatic cancer.
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Pour P, Krueger FW, Althoff J, et al: Cancer of the pancreas induced in the Syrian golden hamster. Am J Pathol 1974; 76: 349–358
Takahashi M, Runge R, Donnelly T, et al: The morphologic and biologic patterns of chemically induced pancreatic adenocarcinoma in Syrian golden hamsters after homologus transplantation. Cancer Lett 1979;7:127–133
Scarpelli DG, Rao MS: Transplantable ductal adenocarcinoma of the Syrian hamster pancreas. Cancer Res 1979;39:452–458
Shindelar WF, Kurman CC: Nitrosamine-induced transplantable pancreatic ductal adenocarcinoma in inbred Syrian Syrian hamsters. J Natl Cancer Inst 1981;67:1093–1100
Ohyama K. Koike D, Okada Y. et al: The enhancement of the antitumor effect of FT-207 coadministrated with uracil on the transplanted ductal adenocarcinoma of the Syrian golden hemster pancreas. Jpn J Cancer Chemother 1982;9:2168–2174
Konishi Y, Takahashi S, Sunagawa M, et al: Heterotransplantation into nude mice of pancreatic carcinoma induced by Nbis (2-hydroxypropyl) nitrosamine in hamsters. Cancer Lett 1978;5:333–337
Townsend CM, Franklin RB, Gelder FB, et al: Development of a transplantable model of pancreatic duct adenocarcinoma. Surgery 1982;92:72–78
Chang BK, Gutman R: Chemotherapy of pancreatic adenocarcinoma, Initial report on two transplantable models in the Syrian hamster. Cancer Res 1982;42:2666–2670
Sindelar WF, Tralka TS, Kurman CC, et al: Demonstration of type-R and type-C virus particles in hamster pancreatic adenocarcinomas. Cancer Lett 1983;18:119–129
Reddy JK, Rao MS: Transplantable pancreatic carcinoma of the rat, Science 1977;198:78–79
Longnecker DS, Lilja HS, French J, et al: Transplantation of azaserine-induced carcinoma of pancreas in rats. Cancer Lett 1979;7:197–202
Rao KN, Takahashi S, Shinozuka H: Acinar cell carcinoma of the rat pancreas grown in cell culture and in nude mice. Cancer Res 1980;40:592–597
Watanabe TK, Hansen LJ, Reddy MK, et al: Differentiation of pancreatic acinal carcinoma cells cultured on rat testicular seminiferous tubular basement membranes. Cancer Res 1984;44:5361–5368
Reddy JK, Kanwar YS, Watanabe TK, et al: Duct-like morphogenesis of longnecker pancreatic acinar carcinoma cells maintained in vitro on seminiferous tubular basement membranes, Cancer Res 1986;46:347–354
Ben-Ze’eve A, Zoller M, Raz A: Differential expression of intermediate filament proteins in metastatic and nonmetastatic variants of the BSp73 Tumor, Cancer Res 1986;46:785–790
Corbett TH, Roberts BJ, Leopold WR, et al: Induction and chemotherapeutic response of two transplantable ductal adenocarcinomas of the pancreas in C57BL/6 mice, Cancer Res 1984;44:717–276
Wilkoff LJ and Dulmadge EA: Sensitivity of proliferating cultured murine pancreatic tumor cells to selected antitumor agents, J Natl cancer Inst 1986;77:1163–1169
Kubota Y, Saito S, Shibuya T, et al: Ultrastructural and cytochemical study of homologously transplanted pancreatic cancers induced by N-nitrosobis (2-hydroxypropyl) amine in Syrian golden hamsters. J Clin Electron Microscopy 1987;20:363–378
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Saito, S., Nishimura, N., Kubota, Y. et al. Establishment and characterization of a cultured cell line derived from nitrosamine-induced pancreatic ductal adenocarcinoma in Syrian golden hamsters. Gastroenterol Jpn 23, 183–194 (1988). https://doi.org/10.1007/BF02799031
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DOI: https://doi.org/10.1007/BF02799031