Skip to main content
SpringerLink
Log in

Activation of sepharose withN,N′-disuccinimidyl carbonate

  • Original Articles
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A method is described for the activation of Sepharose withN,N′-disuccimidyl carbonate. The activated carbonate reacts smoothly with amine-containing ligands yielding stable carbamate derivatives.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Wilchek, M., and Miron, T. (198),Biochem. Int. 4, 629.

  2. Miron, T., and Wilchek, M. (1985),Methods Enzymol, in press.

  3. Raz, A., Meromsky, L., Carmi, P., Karakash, R., Lotan, D., and Lotan, R.EMBO Journal (in press)

  4. Ogura, H., Kobayashi, T., Shimizu, K., Kawabe, K., and Takeda, K.(1979),Tetrahedron Lett. 49, 4745.

    Article  Google Scholar 

  5. Miron, T., and Wilchek, M. (1982),Anal. Biochem. 126, 433.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel

    Meir Wilchek & Talia Miron

Authors
  1. Meir Wilchek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Talia Miron
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wilchek, M., Miron, T. Activation of sepharose withN,N′-disuccinimidyl carbonate. Appl Biochem Biotechnol 11, 191–193 (1985). https://doi.org/10.1007/BF02798475

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02798475

Index Entries

Search

Navigation