Abstract
A novel tumor-detection system consisting of complementmediated cytolytic reaction and an image processing system was developed for the simple and rapid determination of tumor cells.
The present system consists of a CCD image sensor, image memory board, personal computer, and microscope.
When monoclonal antibody 3C4, which is specific to the guinea pig hepatoma L-10, was added to cell suspension, only L-10 cytolysis occurred. Cytolysis caused a decrease in brightness of the cells observed by phase-contrast microscopy. The phase contrast image of the cells before cytolysis was converted to a digitalized signal and stored in computer memory. After cytolysis, a brightness threshold above that of lysed cells was subtracted from the digitalized signal and compared to the signal stored before reaction.
L-10 cells in mixed cell suspension were determined specifically by the system. Measurement time was only 2 sec and overall time, including reaction time, was approximately 30 min. Since this method does not require a cell washing process, automation of the whole system is possible.
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Suzuki, M., Watamabe, N., Tamiya, E. et al. Tumor cell detection method using complement-mediated cytolytic reaction and imaging sensor system. Appl Biochem Biotechnol 15, 191–200 (1987). https://doi.org/10.1007/BF02798448
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DOI: https://doi.org/10.1007/BF02798448