Abstract
The production of lactic acid from glucose has been demonstrated using a CSTR (continuous stirred-tank reactor) with cell recycle. Studies were conducted withLactobacillus delbrueckii at a fermentation temperature of 42°C and a pH of 6.25. A cell density of 140 g dry weight/L and a volumetric productivity of 150 g/L.h, with complete glucose consumption, were obtained. It was not possible to obtain a lactic acid concentration above 60 g/L because of product inhibition. A cell purge was not necessary to maintain high viability bacteria culture or to obtain a steady state. At steady state the net cell growth appeared to be negligible. The specific glucose consumption for cell maintenance was 0.33 g glucose/g cells-h.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- B :
-
Bleed blow/reactor volume (h-1)
- D :
-
Dilution rate (h-1)
- k D :
-
Specific death rate (h-1)
- L :
-
Lactic acid concentration (g/L)
- m :
-
Maintenance coefficient (g/g.h)
- So :
-
Initial glucose concentration (g/L)
- S :
-
Final glucose concentration (g/L)
- t :
-
Time (h)
- μ:
-
Specific growth rate (h-1)
- X :
-
Biomass concentration (g/L)
- Y L/S :
-
Lactic acid yield (g lactic acid produced/g glucose consumed)
- YX/S :
-
Biomass yield (g cells produced/g glucose consumed)
References
I. N. Mark, D. E. Othmer, C. G. Overberger, and G. T. Seaborg (1981), Hydrocarboxylic Acid, Lactic Acid, inEncyclopedia of Chemical Technology, Vol. 13, 3rd Ed. Wiley.
S. S. Stenroos, Y. Y. Linko, and P. Linko,Biotechnol. Lett. 4, 159–164 (1982).
T. B. Vick Roy, H. W. Blanch, and C. R. Wilke,Biotechnol. Lett. 4, 483–488 (1982).
M. R. Friedmann and E. L. Gaden,Biotechnol. Bioeng. 12, 961–974 (1970).
R. W. Stieber and P. Gerhardt,Biotechnol. Bioeng. 23, 535–549 (1981).
L. D. Sortland and C. R. Wilke,Biotechnol. Bioeng. 11, 805–841 (1969).
T. B. Vick Roy, D. K. Mandel, D. K. Dea, H. W. Blanch, and C. R. Wilke,Biotechnol. Lett. 5, 665–670 (1983).
R. Luedeking and E. L. Piret,J. Biochem. Microb. Tech. Eng. 1, 431–459 (1959).
J. H. Janssens, A. Bernard, and R. B. Bailey,Biotechnol. Bioeng. 25, 1–5 (1983).
K. J. Lee, M. Lefebre, D. E. Tribe, and P. L. Rogers,Biotechnol. Lett. 2, 487–492 (1980).
J. R. Postgate, J. R. Crumpton, and J. R. Hunter,J. Gen. Microbiol. 24, 15–24 (1961).
M. M. Bradford,Anal. Biochem. 72, 248–254 (1976).
E. A. Dawes and D. W. Ribbons,Bacteriol. Rev. 28, 126–149 (1964).
H. Stouthamer and C. Bettenhausen,Biochem. Biophys. Acta 301, 53–70 (1973).
A. G. Marr, E. H. Nilson, and D. J. Clark,Ann. NY Acad. Sci. 102, 536–548 (1963).
P. G. Rogers and P. B. Stewart,Arch. Microbiol. 99, 25–46 (1974).
P. J. Reilly, PhD Thesis, 1964, University of Pennsylvania.
A. V. Quirk and J. R. Woodrow,Biotechnol. Lett. 5, 277–282 (1983).
J. P. Belaich, A. Belaich, and P. Simonpietri,J. Gen. Microbiol. 70, 179–185 (1972).
O. M. Neijssel and D. W. Tempest,Arch. Microbiol. 110, 305–311 (1976).
W. Beyeler, P. L. Rogers, and A. Fiechter,Appl. Microbiol. Technol. 19, 277–280 (1984).
T. B. Vick Roy, PhD Thesis, 1983, University of California, Berkeley.
L. A. Rogers and E. O. Whittier,J. Bacteriol. 16, 211–219 (1928).
G. Vinegra-Gonzales and J. Gomez, inFuels and Organic Chemicals from Biomass, 1983, D. Wise, Ed., CRC Press, Boca Raton, FL.
M. R. Friedmann, PhD Thesis, 1967, Columbia University.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ohleyer, E., Blanch, H.W. & Wilke, C.R. Continuous production of lactic acid in a cell recycle reactor. Appl Biochem Biotechnol 11, 317–332 (1985). https://doi.org/10.1007/BF02798444
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02798444
Index Entries
- Lactic acid, production fromLactobacillus delbrueckii
- continuous fermentation, production of lactic acid by
- cross-flow filtration, in continuous lactic acid production
- lactic acid inhibition, during continuous production
- cell concentration during continuous lactic acid production
- broth viscosity, during continuous lactic acid production
- cell recycle, during continuous lactic acid production
- Lactobacillus delbrueckii, continuous production of lactic acid from
- recycle reactor, continuous production of lactic acid in