Skip to main content
SpringerLink
Log in

Scanning electron microscopic observation of porous glass fibers with immobilized glucoamylase

  • Original Article
  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Porous glass fibers with silane-glutaraldehyde immobilized glucoamylase have been examined by Scanning Electron Microscopy (SEM). Partial multilayer coating (“sheeting”) on the fibers’ surfaces has been observed even on gold uncoated samples by using a high resolution SEM. This “sheeting” is attributed to the fiber chemical activation treatment prior to enzyme loading. A 40% reduction of free pore area as a consequence of enzyme attachment is also observed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Reilly, P. J. (1979), inApplied Biochemistry and Bioengineering. vol. 2.Enzyme Technol. Wingard, L. B., Katchalski-Katzir, E., Goldstein, L., eds., Academic, New York, p. 185.

    Google Scholar 

  2. Reilly, P. J. (1985), inStarch Conversion Technology, van Beynum, G. M. A., and Roels, J. A., eds., Marcel Dekker, NY, p. 101.

    Google Scholar 

  3. Pitcher, W. H., Jr., ed. (1980).Immobilized Enzymes for Food Processing, CRC Press, Boca Raton, p. 119.

    Google Scholar 

  4. Messing, R. A. (1985), inComprehensive Biotechnology. vol.2. Moo-Young, M., ed., Pergamon, Oxford, p. 191.

    Google Scholar 

  5. Weetall, H. H. (1985),Trends in Biotechnol.,3, 276.

    Article  CAS  Google Scholar 

  6. Hossain, Md. M., and Do, D. D. (1985),Biotechnol. Bioeng. 27, 842.

    Article  CAS  Google Scholar 

  7. Lee, D. D., Lee, Y. Y., Reilly, P. J., Collins, E. V., Jr., and Tsao, G. T. (1976),Biotechnol. Bioeng. 18, 253.

    Article  CAS  Google Scholar 

  8. Dennis, K. E., Clark, D. S., Bailey, J. E., Cho, Y. K., and Park, Y. H. (1984),Biotechnol. Bioeng.,26, 892.

    Article  CAS  Google Scholar 

  9. Toldrá, F., Jansen, N. B., Tsao, G. T. (1986),Biotechnol. Lett.,8, 785.

    Article  Google Scholar 

  10. Toldrá, F., Jansen, N. B., and Tsao, G. T. (1987),Proc. of the Second World Congress of Food Technol., Barcelona, Spain, March 3–6, p. 259.

  11. Lynn, M. (1975), inImmobilized Enzymes, Antigens, Antibodies and Peptides. vol. 1.Preparation and characterization, Weetall, H. H., ed., Marcel Dekker, NY, p. 9.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute de Agroquímica y Tecnología de Alimentos, Jaime Roig 11, 46010, Valencia, Spain

    J. L. Lequerica & F. Toldrá

  2. Western Regional Research Center, USDA, 800 Buchanan St., 94710, Berkeley, CA, USA

    D. W. Irving

Authors
  1. J. L. Lequerica
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. W. Irving
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Toldrá
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lequerica, J.L., Irving, D.W. & Toldrá, F. Scanning electron microscopic observation of porous glass fibers with immobilized glucoamylase. Appl Biochem Biotechnol 16, 71–77 (1987). https://doi.org/10.1007/BF02798357

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02798357

Index entries

Search

Navigation