Abstract
An extensive selection of immobilized triazine dyes have been examined for their potential as adsorbants for human lymphobalstoid interferon. Procion red HE7B was selected as the most suitable for preparative scale purification. Sepharose-immobilized procion red HE7B is able to bind 105 reference units/mL of interferon from cell supernatants and can be eluted with at least 25-fold purification and 90% yield by a KC1 gradient. Further purification was obtained either by reapplying the eluted interferon after dialysis to the dye column or by gel filtration on Ultrogel AcA 34 after lyophilization and dialysis. The latter procedure gave a final activity of about 106 U/mg protein and approximately 75% recovery of interferon activity.
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Abbreviations
- IFN:
-
interferon
- HuIFN alpha:
-
human lymphoblastoid interferon
- SDS:
-
sodium dodecyl sulfate
- PAGE:
-
polyacrylamide gel electrophoresis
- CPE:
-
cytopathic effect
- SFV:
-
Semliki Forest virus.
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Neame, P.J., Parikh, I. Sepharose-immobilized triazine dyes as adsorbants for human lymphoblastoid interferon purification. Appl Biochem Biotechnol 7, 295–305 (1982). https://doi.org/10.1007/BF02798307
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DOI: https://doi.org/10.1007/BF02798307
Index Entries
- Sepharose-immobilized dyes, in interferon purification
- immobilized dyes, in interferon purification
- triazine dyes, in interferon purification
- dyes, Sepharose-immobilized triazine
- adsorbants, Sepharose-immobilized dyes as
- human lymphoblastoid interferon purification
- lymphoblastoid interferon purification, human
- interferon, human lymphoblastoid
- affinity chromatography