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Effect of purified phospholipases on glucose transport, insulin binding, and insulin action in isolated rat adipocytes

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Abstract

The influence of alterations in phospholipid structure by phospholipase treatment on insulin action and glucose transport in rat adipocytes was studied. It appeared that phospholipase A2 from bee venom caused a breakdown of approximately 50% of phosphotidylcholine without lysis of the cells. Because of this treatment, insulin binding was increased, resulting in an increased sensitivity of glucose transport towards lower insulin concentrations. Moreover, an increased affinity of the transport system for 2-deoxyglucose was observed. Phospholipase C fromClostridium welchii caused complete lysis of adipocytes. Phospholipase A2 from Crotalus adamenteus was without effect.

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Authors and Affiliations

  1. Diabetes Research Laboratory, Department of Endocrinology and Metabolic Diseases, University Hospital, 2333 AA, Leiden, The Netherlands

    H. M. J. Krans

  2. Department of Physiology and Physiological Physics, University Hospital, 2333 AA, Leiden, The Netherlands

    G. de Bruin & W. P. M. van Meerwijk

Authors
  1. Tj. Wieringa
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  2. G. de Bruin
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  3. W. P. M. van Meerwijk
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  4. H. M. J. Krans
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Wieringa, T., de Bruin, G., van Meerwijk, W.P.M. et al. Effect of purified phospholipases on glucose transport, insulin binding, and insulin action in isolated rat adipocytes. Cell Biophysics 14, 261–271 (1982). https://doi.org/10.1007/BF02797402

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  • DOI: https://doi.org/10.1007/BF02797402

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