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Cell Biophysics

, Volume 14, Issue 2, pp 175–185 | Cite as

Scavenging effect of extracts of green tea and natural antioxidants on active oxygen radicals

  • Baolu Zhao
  • Xiaojie Li
  • Rungen He
  • Shujun Cheng
  • Xin Wenjuan
Original Articles

Abstract

With the use of the spin trapping methods, the scavenging effects of the extracts of green tea and other natural foods are studied. In stimulated polymorphonuclear leukocytes (PMN) system, water extract fraction 6 (F6) from green tea and green tea polyphenols (GTP) have the strongest scavenging effect on the active oxygen radicals, much stronger than vitamin C (Vc) and, vitamin E (Ve). Rosemary antioxidants (RA) and Curcumin (Cur) have weaker scavenging effects than Vc, but stronger thanVe. In Fenton Reaction, Cur has the strongest scavenging effect (69%) on hydroxyl radicals. In irradiation, riboflavin system F6(74%) and GTP(72%) have very strong scavenging effects that are weaker than Vc, but much stronger thanVe (23%). With the use of spin probe oxymetry, the oxygen consumption in respiratory burst of stimulated PMN were measured when the antioxidants existed in these systems The results demonstrated that these antioxidants did not affect the respiratory burst of human polymorphonuclear leukocytes stimulated with PMA.

Index Entries

Spin trapping active oxygen radicals antioxidants PMN 

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References

  1. 1.
    Curnutte, J. T., Babior, B. M., and Karnorsky, M. L. (1979),J. Clin. Invest. 63, 637–647.PubMedCrossRefGoogle Scholar
  2. 2.
    Zhao, B-L., Li, X-J. He, R-G, Jia, W-Y, and Xin, W-J (in press).Google Scholar
  3. 3.
    Root, R. K. and Cohen, M. S. (1981),Rev. Infect. Dis. 3, 565–598.PubMedGoogle Scholar
  4. 4.
    Helliwell, B. and Gutteridge, J. M. C. (1984),Biochem. J. 219, 1–14.Google Scholar
  5. 5.
    Mark, J. L. (1987),Science 253, 529–531.Google Scholar
  6. 6.
    Higginson, J. (1967),Natl. Cancer Inst. Monogr. 25, 191–198.PubMedGoogle Scholar
  7. 7.
    Cheng, S-J., Ho, C-T., Lou H-Z., Bao, Y-D., Jian, Y-Z. (1986),Acta Biol. Exp. Sinica 19, 427–431.Google Scholar
  8. 8.
    Colowick, S. P. and Kaplan, N. O. (1984),Methods in Enzymology, vol. 105, Academic Press, New York, NY.Google Scholar
  9. 9.
    Bulttner, G. R. (1982),Superoxide Dismutase, vol. II (Oberley, L. M., ed.) CRC Press, Boca Raton, FL.Google Scholar
  10. 10.
    Fridovich, I. (1975),Ann. Rev. Biochem. 44, 147–159.PubMedCrossRefGoogle Scholar
  11. 11.
    Hassan, H. M. and Fridovich, I. (1984),The Biology and Chemistry of Active Oxygen (Bannister, J. V. and Bannister, W. H., eds.) Elsevier, New York, NY.Google Scholar

Copyright information

© Humana Press Inc. 1989

Authors and Affiliations

  • Baolu Zhao
    • 1
  • Xiaojie Li
    • 1
  • Rungen He
    • 1
  • Shujun Cheng
    • 1
  • Xin Wenjuan
    • 1
  1. 1.Institute of BiophysicsAcademia SinicaBeijingChina

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