Skip to main content

Trace-element determination in individual peripheral blood cells and possible diagnostic applications

Biological Trace Element Research volume 12, pages 351–362 (1987)Cite this article

Abstract

Trace elements are indispensable for the effective and proper functioning of biological systems. Recent years have demonstrated the conspicuous lack of knowledge about trace-element physiology. Establishment of reference values is a very difficult task, requiring the consideration of and compensation for a number of possible simultaneous phenomena.

Peripheral blood has been used in medical diagnosis for a very long time, because, among other things, it is easily accessible. In the search for signs of lack or excess of minerals and trace elements in disease, the interest has been focused mainly on blood plasma or serum. The utilization of blood cells as a marker model is proposed here. The advent of the nuclear microprobe made possible the determination of elemental profiles of individual cells.

The techniques of blood cell separation and preparation for microprobe analysis are presented and discussed. As an example of a possible diagnostic application, a set of reference data from a control group is compared to corresponding data from a group of patients suffering from acute myeloid leukemia.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

43,34 €

Price includes VAT (Finland)
Tax calculation will be finalised during checkout.

References

  1. E. J. Underwood,Trace Elements in Human and Animal Nutrition, 4th ed., Academic, New York, NY, 1977.

    Google Scholar 

  2. G. W. Grime, F. Watt, S. Mann, C. C. Perry, J. Webb, and R. J. P. Williams,Trends in Biochem. Sci. 10, 6 (1985).

    Article  CAS  Google Scholar 

  3. T. B. Johansson, R. Akselsson, and S. A. E. Johansson,Nucl. Instr. Meth. 84, 141 (1970).

    Article  CAS  Google Scholar 

  4. F. Watt, G. W. Grime, and J. Takacs,Nucl. Instr. Meth. B3, 599 (1984).

    Article  Google Scholar 

  5. J. Versieck, Thesis, University of ghent, 1975.

  6. U. Lindh, E. Johansson, and L. Gille,Nucl. Instr. Meth. B3, 631 (1984).

    Article  Google Scholar 

  7. E. Johansson, U. Lindh, and E. Landström,Biol. Trace Elem. Res. 5, 433 (1983).

    CAS  Article  Google Scholar 

  8. D. Brune, U. Lindh, and J. Lorenzen,Nucl. Instr. Meth. 142, 51 (1977).

    Article  CAS  Google Scholar 

  9. U. Lindh and T. Sunde,Nucl. Instr. Meth. B10/11, 703 (1985).

    Article  Google Scholar 

  10. U. Lindh,Anal. Chim. Acta 150, 233 (1983).

    Article  CAS  Google Scholar 

  11. G. J. F. Legge and I. Hammond,J. Microsc. 117, 201 (1979).

    CAS  Google Scholar 

  12. G. V. Iyengar, W. E. Kollmer, and H. J. M. Bowen,The Elemental Composition of Human Tissues and Body Fluids, Verlag Chemie, New York, NY, 1978.

    Google Scholar 

  13. C. Lechene, C. Bronner, and R. G. Kirk,J. Cell Physiol. 90, 117 (1977).

    PubMed  Article  CAS  Google Scholar 

  14. G. D. Blower, D. Phil. Thesis, Oxford, 1983.

  15. G. J. F. Legge,Nucl. Instr. Meth. B3, 561 (1984).

    Article  Google Scholar 

  16. U. Lindh and E. Johansson,Neurotoxicology 4, 177 (1983).

    PubMed  CAS  Google Scholar 

  17. R. Hällgren, K. Svensson, E. Johansson, and U. Lindh,J. Lab. Clin. Med. 104, 893 (1985).

    Google Scholar 

  18. G. Anneren, E. Johansson, and U. Lindh,Acta Paediatr. Scand. 74, 259 (1985).

    PubMed  CAS  Google Scholar 

  19. E. Johansson, U. Lindh, T. Alanen, T. Westermarck, H. Heiskala, P. Santavuori, and I. Elovaara,Med. Biol. 62, 139 (1984).

    PubMed  CAS  Google Scholar 

  20. O. Guillard, A. Piriou, P. Mura, and D. Reiss,Clin. Chem. 25, 1867 (1979)

    PubMed  CAS  Google Scholar 

  21. Y. J. Uemura, Y. Kuno, H. Koyama, T. Yamazaki, and P. Kienle,Nucl. Instr. Meth. 153, 573 (1978).

    Article  CAS  Google Scholar 

  22. T. A. Hall and B. L. Gupta,J. Microsc. 100, 177 (1974).

    PubMed  CAS  Google Scholar 

  23. D. N. Slatkin and K. W. Jones,Nucl. Instr. Meth. 142, 589 (1977).

    Article  Google Scholar 

  24. D. N. Slatkin, A. L. Hanson, K. W. Jones, H. W. Kraner, and J. B. Warren,Nucl. Instr. Meth. 227, 378 (1984).

    Article  Google Scholar 

  25. D. N. Slatkin, R. E. Shroy, and K. W. Jones,Nucl. Instr. Meth. B9, 66 (1985).

    Article  Google Scholar 

  26. L. Grodzins,Neurotoxicology 4, 23 (1983).

    PubMed  CAS  Google Scholar 

  27. L. Grodzins,Nucl. Instr. Meth. 218, 203 (1983).

    Article  CAS  Google Scholar 

Download references

Author information

Affiliations

  1. Department of Physical Biology, Gustaf Werner Institute, Uppsala University, Box 531, S-751 21, Uppsala, Sweden

    Ulf Lindh & Erland Johansson

Authors
  1. Ulf Lindh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Erland Johansson
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lindh, U., Johansson, E. Trace-element determination in individual peripheral blood cells and possible diagnostic applications. Biol Trace Elem Res 12, 351–362 (1987). https://doi.org/10.1007/BF02796691

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02796691

Index Entries

  • Nuclear microprobe
  • micro-PIXE
  • individual blood cells
  • elemental profiles
  • trace elements
  • cell separation