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Pixe analysis of reproductive fluids

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Abstract

An external 3.8-MeV proton beam was employed to induce X-rays in 100-mg pellets of human follicular fluids and in 4–8 mg pellets of spermatozoa. The elements Cl, K, Ca, Fe, Cu, Zn, and Br were quantitatively determined in follicular fluids, whereas the elements, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, and Zn were determined in the spermatozoa. Both samples had high interelement Spearman correlation coefficients. Correlations of elements in the samples were observed with several clinical parameters. The multielemental analysis of spermatozoa can be used to approximate quantities of trace elements inserted into the egg ooplasm at the time of fertilization. These elemental quantities appear to be in the femtogram (10−15) range.

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References

  1. H. Stankovic and D. Mikac-Devic,Clin. Chim. Acta 70, 123 (1976).

    Article  PubMed  CAS  Google Scholar 

  2. B. J. Wood, D. M. Lawrence, and H. G. McGarrigle,Clin. Chim. Acta 123, 329 (1982).

    Article  PubMed  CAS  Google Scholar 

  3. P. A. Pleban and D. Mei,Clin. Chim. Acta 133, 43 (1983).

    Article  PubMed  CAS  Google Scholar 

  4. T. C. Appleton and S. B. Fishel,J. In Vitro Fert. Embryo Trans. 1, 188 (1984).

    Article  CAS  Google Scholar 

  5. R. Akelsson, T. B. Johansson and S. A. E. Johansson,Nucl. Instr. Meth. 84, 141 (1970).

    Article  Google Scholar 

  6. E. T. Williams,Nucl. Instr. Methods 231, 211 (1984).

    CAS  Google Scholar 

  7. Proc. Int. Conf. on Particle-Induced X-Ray Emission and Its Anal. Applic.Nucl. Instr. Meth. 142 (1977), Lund, Sweden, North-Holland Physics Publishing, Amsterdam, The Netherlands.

  8. Proc. Sec. Int. Conf. on Particle-Induced X-Ray Emission and Its Anal. Applic.Nucl. Instr. Meth. 181, (1981), Lund, Sweden, North-Holland Physics Publishing, Amsterdam, The Netherlands.

  9. Proc. Third. Int. Conf. on Particle Induced X-Ray Emission and Its Anal. Applic.,Nucl. Instr Meth. 231 (1984), Heidelberg, West Germany, North-Holland Physics Publishing, Amsterdam, The Netherlands.

  10. Guidelines for Data Acquisition and Data Quality Evaluation in Environmental Chemistry,Anal. Chem. 52, 2242 (1980).

    Google Scholar 

  11. G. S. Hall, N. Roach, U. Simmons, H. Cong, M. Lee, and E. Cummings,J. Radioanal. Chem. 82, 329 (1984).

    Article  CAS  Google Scholar 

  12. T. Kobayashi, T. Martensen, J. Nath, and M. Flavin,Biochem. Biophys. Res. Commun. 81, 1313 (1978).

    Article  PubMed  CAS  Google Scholar 

  13. T. Mann and C. Lutwak-Mann,Male Reproductive Function and Semen, Springer-Verlag, New York, NY, 1981.

    Google Scholar 

  14. H. I. Calvin and G. W. Gooper, inThe Spermatozoon. Maturation, motility, surface properties and comparative aspects, D. W. Fawcett and J M. Bedford (eds.), Urban-Schwarzenberg, Baltimore, Md, 1979.

    Google Scholar 

  15. L. L. Hopkins and H. E. Mohr,Fed. Proc. Fed. Am. Soc. Exp. Biol. 33, 1773 (1974).

    CAS  Google Scholar 

  16. F. W. Sunderman,Am. J. Clin. Pathol. 44, 182 (1965).

    PubMed  Google Scholar 

  17. G. L. Eichhorn,Nature 194, 474 (1962).

    Article  PubMed  CAS  Google Scholar 

  18. W. Wacker, K. Fuwa, and B. L. Vallee,Proc. Natl. Acad. Sci. USA 46, 1298 (1960).

    Article  PubMed  Google Scholar 

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Authors and Affiliations

  1. Dept. of Chemistry, Rutgers, The State University of New Jersey, New Brunswick, 08903, USA

    Gene S. Hall

  2. Dept of OBGYN, University of Medicine and Dentistry, Rutgers Medical School, 08903, New Brunswick, NJ, USA

    Robert M. Shelden

Authors
  1. Gene S. Hall
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  2. Robert M. Shelden
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Hall, G.S., Shelden, R.M. Pixe analysis of reproductive fluids. Biol Trace Elem Res 12, 323–334 (1987). https://doi.org/10.1007/BF02796689

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  • DOI: https://doi.org/10.1007/BF02796689

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