Skip to main content
SpringerLink
Log in

Determination of ruthenium on DNA by XRF

  • Cancer-Related Topics
  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

An X-ray fluorescence (XRF) technique is used to quantitate the binding of [H2O(NH3)5RuII]2+ to DNA. This method is shown to be more sensitive, precise, and convenient than conventional optical absorption (OA) spectroscopy, differential pulse voltammetry (DPV), or atomic absorption (AA) techniques. X-ray fluorescence is insensitive to the oxidation state or coordination environment of Ru and so can be used to determine total Ru. The minimum detectable amount of Ru is 10 ng in 1 h of counting time, using a 100-mCi125I source. The specific advantages of the XRF method over the conventional methods are outlined.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. M. J. Clarke, inMetal Ions in Biological Systems, vol. 11, H. Sigel, ed., Marcel Dekker, New York and Basel, 1980, pp. 231–283.

    Google Scholar 

  2. M. J. Clarke, inInorganic Chemistry in Biology and Medicine, A. E. Martell, ed., Am. Chem. Soc. Washington, DC, 1980, pp. 157–180.

    Google Scholar 

  3. M. J. Clarke, inMetal Chemotherapeutic Agents, Am. Chem. Soc. Washington, DC, 1983, pp. 157–180.

    Google Scholar 

  4. M. P. Hacker, E. B. Douple, and I. H. Krakoff,Platinum Coordination Complexes in Cancer Chemotherapy, Nijhaff, Boston, MA, 1983.

    Google Scholar 

  5. S. C. Srivastava, P. Richards, G. E. Meinken, P. Som, H. L. Atkins, M. S. Larson, L. Grunbaum, J. S. Rasey, M. Dowling, M. J. Clarke, inRadiopharm. 2, Proc. Int. Symp. 2nd., J. A. Sorenson, ed., Soc. Nucl. Med., Inc. New York, NY, 1979, pp. 265–274.

    Google Scholar 

  6. K. Mizukawa, G. Yamamoto, T. Tamai, M. Tanabe, M. Yamoyto,Radioisotopes 21, 19 (1978).

    Google Scholar 

  7. M. J. Clarke, M. Buchbinder, A. D. Kelman,Inorgan. Chim. Acta 27, 287 (1978).

    Article  Google Scholar 

  8. J. A. Plambeck,Electroanalytical chemistry, Wiley-Interscience, New York, NY, 1982, pp. 256–328.

    Google Scholar 

  9. M. J. Clarke,J. Am. Chem. Soc. 100, 5068 (1978).

    Article  CAS  Google Scholar 

  10. L. Wielopolski, R. Zhang, S. H. Cohn,Description of an XRF System for Multielemental Analysis, Biolog. Tr. El. Res. 13, 345 (1987).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medical Department, Brookhaven National Laboratory, 11973, Upton, LI, New York

    L. Wielopolski, R. Zhang & S. H. Cohn

  2. Department of Chemistry, Boston College, 02167, Chestnut Hill, MA

    M. J. Clarke

Authors
  1. L. Wielopolski
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. J. Clarke
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. H. Cohn
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wielopolski, L., Zhang, R., Clarke, M.J. et al. Determination of ruthenium on DNA by XRF. Biol Trace Elem Res 13, 283–290 (1987). https://doi.org/10.1007/BF02796639

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02796639

Index Entries

Search

Navigation