Summary
An adult male African green monkey (Cercopithecus aethiops) with an undifferentiated carcinoma, probably originating from the nasal mucosa, was received from the Akron, Ohio, zoo. Cultivation of this tumor in vitro resulted in a mixture of fibroblastic and epithelial cells which was subsequently separated using differential trypsinization. The neoplastic nature of the cultured epithelial cells was verified by their ability to transplant into athymic nude, or antithymocyte serum-treated mice, where poorly differentiated carcinomas were produced, and cultures of the tumors that arose in nude mice were morphologically similar to pretransplantation cultures. Early cultures showed a normal male karyotype characteristic of the species; however, in long-term cultures, a clearly defined, small submetacentric Y chromosome was not observed. Electron microscopic examination of tumor tissue and cultured tumor cells revealed desmosomes and the presence of cytoplasmic (keratin-type) fibrils, which tended to be organized around the nucleus. In addition to the keratin-type fibrils, the cultured tumor cells also contained a large amount of cytoplasmic inclusion material that may represent keratohyalin granules. There was no evidence of a viral association with tumor material or cultured cells. The cultures were susceptible to infection by vesicular stomatitis virus,Herpesvirus hominis type 1, andH. saimiri, but were resistant to the Epstein-Barr virus.
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This work was supported in part by contracts N01-CP-53509 and N01-CP-43209 of the Virus Cancer Program, National Cancer Institute.
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Neubauer, R.H., Rabin, H., Arnstein, P. et al. Characterization of a spontaneous undifferentiated carcinoma from an African green monkey (Cercopithecus aethiops). In Vitro Cell.Dev.Biol.-Plant 12, 533–539 (1976). https://doi.org/10.1007/BF02796496
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DOI: https://doi.org/10.1007/BF02796496