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Growth inhibitors in plasma derived human serum

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Summary

It was reported previously that plasma derived human serum (PDS) inhibited the growth of cells established from malignant human breast tissues and the MCF-7 cell line but did not inhibit the growth of cells from nonmalignant mammary tissues, including the HBL-100 cell line. Plasma derived human serum was fractionated in the current study by molecular sieve chromatography on Sephadex G-100 in an effort to characterize the factor(s) responsible for inhibition. Plasma derived human serum contained several growth inhibitory fractions, which were designated G-1, G-2, G-3, and G-4. The G-1 was associated with the lipoproteins and immunoglobulins of serum. The lipid portion of G-1 inhibited the growth of both MCF-7 and HBL-100 cells, whereas the protein fraction contained a low activity factor directed against MCF-7 cells only. The G-2 also inhibited MCF-7 cell growth at a low specific activity and was separated in the serum albumin fraction. The MCF-7 inhibitory activity in the G-3 fractions from individual donors fluctuated with the level of activity in the starting sera. The cell specific G-3 components were purified further by Sephadex G-100 superfine chromatography and gel electrophoresis. A tentative molecular weight of 50,000 was assigned to the G-3 inhibitor. The G-4 fraction consisted of small molecular weight materials migrating in advance of the column volume, which inhibited the growth of both cell lines.

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Authors and Affiliations

  1. Microbiology Program, The Pennsylvania State University, 16802, University Park, Pennsylvania

    Dennis A. Pigott, Margaret A. Grimaldi, Marie L. Dell’Aquila & Edwin V. Gaffney

Authors
  1. Dennis A. Pigott
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  2. Margaret A. Grimaldi
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  3. Marie L. Dell’Aquila
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  4. Edwin V. Gaffney
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Additional information

This investigation was supported by Grant PDT-140 from the American Cancer Society, Inc., and PHS Grant CA30284 awarded by the National Cancer Institute, Bethesda, Maryland.

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Pigott, D.A., Grimaldi, M.A., Dell’Aquila, M.L. et al. Growth inhibitors in plasma derived human serum. In Vitro Cell.Dev.Biol. -Plant 18, 617–625 (1982). https://doi.org/10.1007/BF02796394

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  • DOI: https://doi.org/10.1007/BF02796394

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