Summary
Ornithine decarboxylase activity was measured during organogenesis in rat embryos grown in utero and whole rat conceptuses maintained in an in vitro culture system. Ornithine decarboxylase levels in vivo showed a distinct peak at embryonic age 10.5 d. Despite identical morphology, protein content, crown rump length and numbers of somites cultured embryos displayed a different developmental pattern and possessed less than half the ODC activity of that in vivo. The data suggest that the normal embryonic programming of ODC activity is significantly altered by the culture environment and that further biochemical comparisons of embryos growing in utero and in vitro may be required to evaluate properly the applicability of this technique to detailed studies of teratogenesis and developmental biology.
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This work was supported by NIH-5-507-RR5359-17 and a 1980 Research Starter Grant from the Pharmaceutical Manufacturers Association Foundation.
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Huber, B.E., Brown, N.A. Developmental patterns of ornithine decarboxylase activity in organogenesis phase rat embryos in culture and in utero. In Vitro Cell.Dev.Biol. -Plant 18, 599–605 (1982). https://doi.org/10.1007/BF02796391
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DOI: https://doi.org/10.1007/BF02796391