Summary
Testosterone stimulated, at the concentration of 10−7 M and independently of other hormones, the accumulation of polysomal poly(A)-containing RNA (mRNA) in cultured explants of rat ventral prostate and concomitantly also protein synthesis. The hormone-induced accumulation of polysomal mRNA, which reached its maximum at 24 h after testosterone addition, paralleled the preferential labeling of high molecular weight RNA demonstrable with the electrophoretic analysis of the double-isotope labeled RNA after a short pulse (30 min). These findings are consistent with the idea that testosterone activated the synthesis of precursor mRNA leading to an increased amount of polysomal mRNA and eventually an activated protein synthesis.
The synthesis and maturation of rRNA appeared to proceed even in the absence of testosterone, which is in contrast to the in vivo findings on castrated rats. This partial uncoupling of RNA synthesis from androgenic control may account for the slow and less marked hormonal response found in protein synthesis and glucose metabolism in cultured explants from normal animals.
Because of the lack of uniformity in the structure, routine light microscopic control to assess the viability of cultured explants was found to be a prerequisite for successful biochemical work on prostate culture.
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Parker, M. G.; Scrace, G. T.; Mainwaring, W. I. P. Testosterone regulates the synthesis of major proteins in rat ventral prostate. Biochem. J. 170: 115–121; 1978.
Hiremath, S. T.; Wang, T. Effect of testosterone on RNA sequence complexity in rat prostate. Biochem. Biophys. Res. Commun. 89: 1200–1205; 1979.
Yamanaka, H.; Kirdani, R. Y.; Saroff, J.; Murphy, G. P.; Sandberg, A. A. Effects of testosterone and prolactin on rat prostate weight, 5α-reductase, and arginase. Am. J. Physiol. 229: 1102–1109; 1975.
Aumüller, G. Handbuch der Mikroskopischen Anatomie des Menschen VII/6, Prostate Gland and Seminal Vesicles. New York: Springer-Verlag; 1979: 141–142.
Boyns, A. R.; Griffiths, K.; Pierrepoint, C. G.; Peeling, W. B. Prolactin and the prostate. Goland, M. ed. Normal and abnormal growth of the prostate. Springfield, IL: Charles C. Thomas; 1975: 431–444.
Johansson, R. Effect of prolactin, growth hormone and insulin on the uptake and binding of dihydrotestosterone to the cultured rat ventral prostate. Acta Endocrinol. (Copenh.) 81: 854–864; 1976.
Resnick, M. J.; Walvoord, P. J.; Grayhack, J. T. Effect of prolactin on testosterone uptake by the perfused canine prostate. Surg. Forum 25: 70–72; 1974.
Grayhack, J. T.; Lebowitz, J. M. Effect of prolactin on citric acid of lateral lobe of prostate of Sprague-Dawley rat. Invest. Urol. 5: 87–94; 1967.
Johansson, R. RNA, protein and DNA synthesis stimulated by testosterone, insulin and prolactin in the rat ventral prostate culture in chemically defined medium. Acta Endocrinol. (Copenh.) 80: 761–774; 1975.
Lostroh, A. J. Regulation by testosterone and insulin of citrate secretion and protein synthesis in explanted mouse prostates. Proc. Natl. Acad. Sci. USA 60: 1312–1318; 1968.
Santti, R. S.; Johansson, R. Some biochemical effects of insulin and steroid hormone on the rat prostate in organ culture. Exp. Cell. Res. 77: 111–120; 1973.
Trowell, O. A. The culture of mature organs in a synthetic medium. Exp. Cell. Res. 16: 118–147; 1959.
Palmiter, R. Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undegraded polysomes and messenger ribonucleic acid. Biochemistry 13: 3606–3615; 1974.
Edström, J-E.; Rydlander, L. Identification of cytoplasmic RNA from individual Balbiani rings. Biol. Zbl. 95: 521–530; 1977.
Molloy, G. R.; Jelinek, W.; Salditt, M.; Darnell, J. E. Arrangement of specific oligonucleotides within poly(A) terminated HnRNA molecules. Cell 1: 43–53; 1974.
Edström, J-E.; Ranguay, R. Cytoplasmic ribonucleic acids with messenger characteristics in salivary gland cells ofChironomus tentans. J. Mol. Biol. 84: 569–583; 1974.
Aures, D.; Clark, W. G. A rotating diffusion chamber for C1402 determination as applied to inhibitor studies on mouse mast cell tumor histidine decarboxylase. Anal. Biochem. 9: 35–47; 1964.
Isotalo, A.; Santti, R. S. Effect of inhibitors of RNA and protein synthesis on the prostatic response to testosterone. Biochim. Biophys. Acta 277: 595–605; 1972.
Mans, R. J.; Novelli, G. D. A convenient, rapid and sensitive method for measuring the incorporation of radioactive amino acids into protein. Biochem. Biophys. Res. Commun. 3: 540–543; 1960.
Härkönen, P.; Kuosa, A.; Santti, R. S. Studies on the mechanism of testosterone action on glucose metabolism in the rat ventral prostate. J. Steroid Biochem. 6: 1405–1413; 1975.
Kuosa, A. Androgen-induced accumulation of polysomal poly(A)-containing RNA in the ventral prostate of the rat. Acta Endocrinol. (Copenh.). In press; 1982.
Lasnitzki, I. Action and interaction of hormones and 3-methylcholanthrene on the ventral prostate gland of the rat in vitro. I. Testosterone and methylcholanthrene. J. Natl. Cancer Inst. 35: 339–348; 1965.
Baulieu, E. E.; Lasnitzki, I.; Robel, P. Metabolism of testosterone and action of metabolites on prostate glands grown in organ culture. Nature 219: 1155–1156; 1968.
Gittinger, J. W.; Lasnitzki, I. The effect of testosterone and testosterone metabolites on the fine structure of the rat prostate gland in organ culture. J. Endocrinol. 52: 459–464; 1972.
Ichihara, I.; Santti, R. S.; Pelliniemi, L. J. Effects of testosterone, hydrocortisone and insulin on the fine structure of the epithelium of rat ventral prostate in organ culture. Z. Zellforsch. 143: 425–438; 1973.
Wang, T. Y.; Loor, R. M. Testosterone-activated RNA synthesis in isolated prostate nuclei. J. Steroid Biochem. 10: 299–304; 1979.
Parker, M. G.; Mainwaring, W. I. P. Effects of androgens on the complexity of poly(A)RNA from rat prostate. Cell 12: 401–407; 1977.
Parker, M. G.; Mainwaring, W. I. P. Androgenic regulation of poly(A)-containing RNA sequences in rat ventral prostate. J. Steroid Biochem. 9: 455–460; 1978.
Parker, M. G.; Scrace, G. T. The androgenic regulation of abundant mRNA in rat ventral prostate. Eur. J. Biochem. 85: 399–406; 1978.
Alfheim, I. 5α-Dihydrotestosterone and the synthesis of RNA in the rat ventral prostate in vitro. II. Acta Endocrinol. (Copenh.) 73: 189–195; 1973.
Isotalo, A.; Santti, R. S. Changes in labelling of ribonucleic acid species fractionated from the rat ventral prostate in response to testosterone. Acta Endocrinol. (Copenh.) 78: 401–416; 1975.
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This work was supported by the Finnish Cultural Foundation (Suomen Kulttuurirahasto) and the Research and Science Foundation of Lääke OY (Lääke OY:n Tutkimus- ja Tiedesäätiö).
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Kuosa, A., Lahtonen, R., Lähdetie, J. et al. Androgenic control of polysomal poly(A)-containing RNA in the cultured rat ventral prostate. In Vitro Cell.Dev.Biol. -Plant 18, 585–592 (1982). https://doi.org/10.1007/BF02796389
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DOI: https://doi.org/10.1007/BF02796389