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Laminin provides a better substrate than fibronectin for attachment, growth, and differentiation of 1003 embryonal carcinoma cells

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Summary

Culture of cells in hormonally defined media has allowed (a) the demonstration of physiological responses from cells usually unable to express them in vitro and (b) the study of the effects on growth and differentiation of diffusible factors and attachment factors. The embryonal carcinoma line 1003 forms multidifferentiated tumors in vivo but is unable to differentiate in vitro when grown in serum-containing medium. In a defined medium containing insulin, transferrin, selenium, and fibronectin as attachment factors, 1003 cells grow for several generations and differentiate into neurons and embryonic mesenchyme (Darmon et al., 1981, Dev. Biol. 85: 463–473). In the present work the effects of fibronectin and laminin were compared. In the presence of laminin the cells attached and spread better, grew faster, and could be plated at lower densities. Neurite extension was also better under these conditions and most importantly, it was found that laminin induced an important formation of muscular tissue when the cells had been seeded at low densities. Multinucleated myotubes could be stained with antibodies directed against embryonic muscular myosin. Coating the dishes with polylysine or adding FGF or serum-spreading factor to the medium allowed growth of low-density cultures with fibronectin instead of laminin but muscular differentiation was not detected under these conditions. Addition of fibronectin to laminin-containing medium did not inhibit muscular differentiation.

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Authors and Affiliations

  1. Département de Biologie Moléculaire, Institut Pasteur, 25, rue du Dr. Roux, 75025, Paris, France

    Michel Y. Darmon

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  1. Michel Y. Darmon
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Additional information

Presented in the symposium on Plant and Animal Physiology in Vitro at the 33rd Annual Meeting of the Tissue Culture Association, San Diego, California, June 6–10, 1982.

This research was supported in part by grants from the “Centre National de la Recherche Scientifique” (LA 269), the “Délégation Générale à la Recherche Scientifique et Technique,” the Fondation pour la Recherche Médicale Française,” the “Institut National de la Santé et de la Recherche Medicale,” the “Ligue Nationale Française centre le Cancer,” and the “Fondation André Meyer.”

This symposium was supported in part by the following organizations: Bellco Glass, Inc., California Branch of the Tissue Culture Association, Collaborative Research, Hana Media, Hybridtech, K C Biological, Inc., and Millipore Corporation.

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Darmon, M.Y. Laminin provides a better substrate than fibronectin for attachment, growth, and differentiation of 1003 embryonal carcinoma cells. In Vitro Cell.Dev.Biol.-Plant 18, 997–1003 (1982). https://doi.org/10.1007/BF02796374

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  • DOI: https://doi.org/10.1007/BF02796374

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