Summary
A small hot wire device for cutting plastic culture ware can be constructed of steel rod, brass screws, nichrome wire and acrylic plastic sheeting and tubing. The nichrome wire is heated using a variable power transformer. Four sequential cuts are made in the culture flask bottom and the bottom separated from the remainder of the flask. Cultures can be stained, air-dried and cover slips affixed with PVP or epoxy resin. This method of cutting culture ware avoids the formation of small bits of polystyrene generated by rotating discs or saws.
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References
Fox, C. H., M. Cottler-Fox, and K. K. Sanford. 1974. Rapid staining and preservation of cells grown in plastic tissue culture flasks. Stain Technol. 49: 105–107.
MacCallum, D. B. 1962. Staining and mountingin situ of cell monolayers grown in plastic dishes. Stain Technol. 37: 129–131.
Ores, R. 1971. Advantages of epoxy resin as a mounting medium for light microscopy. Stain Technol. 46: 315–317.
Palevitz, B., and E. H. Newcombe. 1970. A study of sieve element starch using sequential enzymatic digestion and electron microscopy. J. Cell Biol. 383–398.
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McKelvey, S.W., Singer, P.A., Lillie, J.H. et al. A hot wire device for cutting tissue culture flasks. In Vitro Cell.Dev.Biol.-Plant 12, 845–846 (1976). https://doi.org/10.1007/BF02796370
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DOI: https://doi.org/10.1007/BF02796370