Abstract
An experimental procedure and method of analysis are presented for calibration of a thin-beam force transducer. The beam transducer can be produced and calibrated with a minimum coefficient of 10 ng (10−5 dyne) force per micron (10−4 cm) deflection, i.e.,k B∼0.1 dyne/cm. Since beam deflections on the order of 0.1 μm can be detected, forces of a few nanograms can be resolved. Such forces are common in mechanical experiments on microscopic bodies, e.g., biological cells, artificial membrane capsules, droplets, etc.
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References
Cole, K. S. (1932),J. Cell. Comp. Physiol. 1, 1.
Yoneda, M. (1964),J. Exp. Biol. 41, 893.
Hiramoto, Y. (1963),Exp. Cell Res. 32, 59.
Evans, E. A., and Hochmuth, R. M. (1978), “Mechano-Chemical Properties of Membranes,” inCurrent Topics in Membranes and Transport, Vol. X, Kleinzeller and Bronner, eds., Academic Press, pp. 1–64.
Evans, E. A., and Skalak, R.,Mechanics and Thermodynamics of Biomembranes, C. R. C. Press, Boca Raton, Fla., 1980.
Evans, E. A., and Waugh, R. (1977),J. Colloid Interface Sci. 60, 286.
Landau, L. D., and Lifshitz, E. M. (1970),Theory of Elasticity, translated by J. B. Sykes and W. H. Reid, Pergamon Press, Oxford.
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Evans, E.A., Kwok, R. & McCown, T. Calibration of beam deflection produced by cellular forces in the 10−9–10−6 gram range. Cell Biophysics 2, 99–112 (1980). https://doi.org/10.1007/BF02795837
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DOI: https://doi.org/10.1007/BF02795837