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Clinical utility of serodiagnosis of tuberculosis

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Abstract

The use of TB-ELISA tests as a diagnostic tool offer a lot of scope in early diagnosis of serious forms of childhood tuberculosis. The characteristics of these tests have improved with the availability of purified and recombinant antigens and competition assays using monoclonal antibodies. Lower antibody titres toM. tuberculosis specific antigens in children and the presence of ‘natural exposure’ antibodies make the interpretation of these tests difficult at times. Caution must be exercised in interpreting their results due to problems inherent on currently available methods of TB-immunodiagnosis. The selection of best combination of antigens for serology, prospective clinical trials comparing success rate of serology with the standard different diagnostic procedures are required.

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References

  1. Wilkins EGL, Ivanyi J. Potential value of serology for diagnosis of extrapulmonary tuberculosis.Lancet 1990; 336: 641–644.

    Article  PubMed  CAS  Google Scholar 

  2. Ivanyi J, Bothamley GH, Jackett PS. Immunodiagnostic assays for tuberculosis and leprosy.Br Med Bull 1988; 44: 635–649.

    PubMed  CAS  Google Scholar 

  3. Singh M, Andersen AB, MacCarthy JEGet al. TheM. tuberculosis 38 KDa antigen overproduction inEscherichia coli, purification and characterisation.Gene 1992; 117: 53–60.

    Article  PubMed  CAS  Google Scholar 

  4. Young DB, Kaufman SHE, Hermans PWM, Thole JER. Mycobacterial protein antigens: a compilation.Mol Microbiol 1992; 6: 133–145.

    Article  PubMed  CAS  Google Scholar 

  5. Chan SL, Reggiardo Z, Daniel TMet al. Serodiagnosis of tuberculosis using an ELISA with antigen 5 and a haemagglutination assay with glycolipid antigens.Am Rev Respir Dis 1990; 142: 385–390.

    PubMed  CAS  Google Scholar 

  6. Verbon A, Hartskeerl RA, Schuitema Aet al. Characterisation ofM. tuberculosis 14 K antigen.J Bacteriol 1992; 174: 1352–1359.

    PubMed  CAS  Google Scholar 

  7. Lee BY, Hefta SA, Brennan PJ. Characterisation of the major membrane proteins of virulentMycobacterium tuberculosis.Infection and Immunity 1992; 60: 2066–2074.

    PubMed  CAS  Google Scholar 

  8. Chanteau S, Glaziou P, Chansin R. Assessment of diagnostic value of the active PGI-Tb1, its synthetic neoglycocnjugate PGL TBO and the sulfolipid IV antigens for the serodiagnosis of tuberculosis.Int J Lepr 1992; 60: 1–7.

    CAS  Google Scholar 

  9. Daleine G, Lagrange PH. Preliminary evaluation ofMycobacterium tuberulosis lipo-oligosaccharide (LOS) antigen in the serological diagnosis of tuberculosis in HIV seropositive and seronegative patients.Tubercle and Lung Disease 1995; 76: 234–239.

    Article  PubMed  CAS  Google Scholar 

  10. Khanolkar Young S, Kolk AHJ, Andersen ABet al. Results of the thrid immunology of leprosy/immunology of tuberculosis anti-mycobacterial monoclonal antibody workshop.Infect Immun 1992; 60: 3925–3927.

    Google Scholar 

  11. Rosen EU. The diagnostic value of an enzyme-linked immunosorbent assay using adsorbed mycobacterial sonicates in children.Tubercle 1990; 71: 127–130.

    Article  PubMed  CAS  Google Scholar 

  12. Barrera L, Miceli J, Ritacco Vet al. Detection of circulating antibodies to purified protein derivative by enzyme-linked immunosorbent assay: its potential for the rapid diagnosis of tuberculosis.Pediatr Infect Dis J 1989; 8: 763–767.

    Article  PubMed  CAS  Google Scholar 

  13. Zheng YJ, Wang RH, Lin YZ, Daniel TM. Clinical evaluation of the diagnostic value of measuring IgG antibody to 3 mycobacterial antigen preparations in the capillary blood of children with tuberculosis and control subjects.Tubercle and Lung Disease 1994; 75: 366–370.

    Article  PubMed  CAS  Google Scholar 

  14. Delacourt C, Gobin J, Gaillard JLet al. Value of ELISA using antigen 60 for the diagnosis of tuberculosis in children.Chest 1993; 104: 393–398.

    Article  PubMed  CAS  Google Scholar 

  15. Chandramuki A, Bothamley GH, Brennan PJ, Ivanyi J. Levels of antibody to defined antigens ofMycobacterium tuberculosis in tuberculous meningitis.J Clin Microbiol 1989; 27: 821–825.

    PubMed  CAS  Google Scholar 

  16. Vaidya A, Wagle N, Joshi N, Merchant SM. Detection of tubercle antigen in cerebrospinal fluids by ELISA for diagnosis of tuberculous meningitis.Indian J Pediatr 1990; 57: 679–683.

    Article  PubMed  Google Scholar 

  17. Alde SLM, Pinasco HM, Pelosi FRet al. Evaluation of an enzyme-linked immunosorbent assay (ELISA) using an IgG antibody toMycobacterium tuberculosis antigen 5 in the diagnosis of active tuberculosis in children.Am Rev Respir Dis 1989; 139: 748–751.

    PubMed  CAS  Google Scholar 

  18. Mathai A, Radhakrishnan VV, Mohan PK, Sehagal S. ELISA of IgG antibody toMycobacterium tuberculosis antigen 5, PPD in CSF in tuberculous meningitis.Indian J Med Res 1990; 91: 425–430.

    PubMed  CAS  Google Scholar 

  19. Rajajee S, Raja A. Antibody to PPD, H37RV and antigen 5 in tuberculous meningits.J Trop Pediatr 1991; 37: 266–267.

    PubMed  CAS  Google Scholar 

  20. Mahadevan S. Childhood neurotuberculosis: Diagnostic aspect. Ph.D. Thesis. University of Pondicherry 1994; 86–91.

  21. Bothamley GH. Serological diagnosis of tuberculosis.Eur Respir J 1995; 8 (Suppl 20): 676–688.

    Google Scholar 

  22. Bothamley GH, Udani P, Rudd R, Festenstein F, Ivanyi J. Humoral response to defined epitopes of tubercle bacilli in adult pulmonary and child tuberculosis.Eur J Clin Microbiol Infect Dis 1988; 7: 639–645.

    Article  PubMed  CAS  Google Scholar 

  23. Bothamley GH, Rudd R, Festenstein F, Ivanyi J. Clinical value of the measurement ofMycobacterium tuberculosis specific antibody in pulmonary tuberculosis.Thorax 1992; 47: 270–275.

    Article  PubMed  CAS  Google Scholar 

  24. Sada E, Brennan PJ, Herrera T. Evaluation of lipoarabinomannan for the serological diagnosis of tuberculosis.J Clin Microbiol 1990; 28: 2587–2590.

    PubMed  CAS  Google Scholar 

  25. Sada E, Ruiz-Palacios GM, Lopez-Vidal Yet al. Detection of mycobacterial antigens in cerebrospinal fluid of patients with tuberculous meningitis by enzyme-linked immunosorbent assay.Lancet 1983; 11: 651–652.

    Article  Google Scholar 

  26. Bothamley GH, Rudd RM. Clinical evaluation of a serological assay using monoclonal antibody (TB 72) to the 38 KDa antigen ofMycobacterium tuberculosis.Eur Respir J 1994; 7: 240–246.

    Article  PubMed  CAS  Google Scholar 

  27. Bothamley GH, Batra H, Ramesh V, Chandramuki A, Ivanyi J. Serodiagnostic value of the 19 kilodaltion antigen ofMycobacterium tuberculosis in Indian patients.Eur J Clin Microbiol Infect Dis 1992; 11: 6–10.

    Article  Google Scholar 

  28. Kadival G, Samuel AM. IAEA sponsored multicentred trial on immunodiagnosis for TB meningitis.Personal Communication 1991.

  29. Mahadevan S. Advances in diagnosis of tuberculosis.Indian J Pediatr 1996; 63: 153–157.

    PubMed  CAS  Google Scholar 

  30. Chandramuki A, Allen PRJ, Keen M, Ivanyi, J. Detection of mycobacterial anitgen and antibodies in the cerebrospinal fluid of patients with tuberculous meningitis.J Med Microbiol 1985; 20: 239–247.

    PubMed  CAS  Google Scholar 

  31. Wilkins EGL, Bothamley GH, Jackett PS. A rapid, simple ELISA to measure antibody to individual epitopes in the serodiagnosis of tuberculosis.Eur J Clin Microbiol Infect Dis 1992; 10: 559–563.

    Article  Google Scholar 

  32. Pierre C, Olivier C, Lecossier Det al. Diagnosis of primary tuberculosis in children by amplification and detection of mycobacterial DNA.Am Rev Respir Dis 1993; 147: 420–424.

    PubMed  CAS  Google Scholar 

  33. Delacourt C, Poveda JD, Chureau Cet al. Use of polymerase chain reaction for improved diagnosis of tuberculosis in children.J Pediatr 1995; 126: 703–709.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Pediatrics, Jawaharlal Institute of Postgraduate Medical Education and Research (JIPMER), 605006, Pondicherry

    S. Mahadevan (Associate Professor)

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  1. S. Mahadevan
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Mahadevan, S. Clinical utility of serodiagnosis of tuberculosis. Indian J Pediatr 64, 97–103 (1997). https://doi.org/10.1007/BF02795786

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