Skip to main content
Log in

Zinc, ethanol, and lipid peroxidation in adult and fetal rats

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

Studies were performed on adult and fetal rats receiving either a zinc-deficient (<0.5 ppm) diet and/or ethanol (20%) throughout pregnancy. Liver zinc levels were depressed in fetuses exposed toin utero zinc deficiency, but brain zinc levels were unchanged. Ethanol had no effect on the concentration of zinc in the several fetal and adult tissues studies. Lipid peroxidation, as measured by endogenous levels of malondialdehyde (MDA) increased following food restriction, zinc improverishment, and alcoholism in adult and fetal livers, but not in fetal brains. Generally, levels of MDA were highest when both zinc deficiency and the ingestion of alcohol occurred concurrently. Glutathione (GSH) was depressed by zinc restriction in several adult and fetal tissues, but not in the fetal brain. Ethanol alone had no effect on GSH levels. The activity of the enzyme glutathione peroxidase (GSH-Px) was not changed in either organism by alcohol or zinc deficiency.

Overall, the data point to increased lipid peroxidation in maternal and fetal rat tissues following zinc depletion and/or treatment with alcohol and draw attention to the apparent vulnerability of the fetal liver toin utero alcoholism. By contrast, the fetal brain seems to be especially resistant to alcohol and zinc-related lipoperoxidation. An association is suggested between the increased lipoperoxidation accompanying zinc deficiency and reduced levels of GSH, but this does not appear to relate to changes in the activity of GSH-Px. A similar relationship is not evident with respect to the increased levels of MDA in fetal and adult livers following chronic alcohol intoxication. A possible basis for the zinc-GSH interaction is discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. M. Chvapil,Med. Clin. N. Am. 60, 799 (1967).

    Google Scholar 

  2. W. J. Bettger and B. L. O'Dell,Life Sci. 28, 1425 (1981).

    Article  PubMed  CAS  Google Scholar 

  3. I. E. Dreosti, inMechanisms of Alcohol Damage in Utero, Ciba Symposium 105, Pittman, London, 1984, pp. 103–115.

    Google Scholar 

  4. J. F. Sullivan, M. M. Jetton, H. K. Hahn, and R. E. Burch,Am. J. Clin. Nutr. 33, 51 (1980).

    PubMed  CAS  Google Scholar 

  5. I. E. Dreosti, I. R. Record, and S. J. Manuel,Biol. Trace Elem. Res. 7, 103 (1985).

    Article  CAS  Google Scholar 

  6. A. Valenzuela, V. Fernandez, and L. A. Videla,Toxicol. Appl. Pharmacol. 70, 87 (1983).

    Article  PubMed  CAS  Google Scholar 

  7. L. A. Videla and A. Valenzuela,Life Sci. 31, 2395 (1982).

    Article  PubMed  CAS  Google Scholar 

  8. R. E. Litov, D. H. Irvin, J. E. Downey, and A. L. Tappel,Lipids 13, 305 (1978).

    Article  PubMed  CAS  Google Scholar 

  9. S. Shaw, E. Yayatilleke, W. A. Ross, E. R. Gordon, and C. Lieber,J. Lab. Clin. Med. 98, 417 (1981).

    PubMed  CAS  Google Scholar 

  10. M. V. Torrielli, L. Gabriel, and M. U. Dianzani,J. Pathol. 126, 11 (1978).

    Article  PubMed  CAS  Google Scholar 

  11. H. Speisky, D. Bunout, H. Orrego, H. G. Giles, A. Gunasekara, and Y. Israel,Res. Comm. Chem. Pathol. Pharmacol. 48, 77 (1985).

    CAS  Google Scholar 

  12. H. W. Sippel,Acta Pharmacol. Toxicol. 53, 135 (1983).

    Article  CAS  Google Scholar 

  13. C. Guerri and S. Grisolia,Pharmacol. Biochem. Behav. 13, 53 (1980).

    PubMed  CAS  Google Scholar 

  14. H. Speisky, A. Macdonald, G. Giles, H. Orrego, and Y. Israel,Biochem. J. 225, 565 (1985).

    PubMed  CAS  Google Scholar 

  15. Y. Kera, S. Komura, Y. Ohbora, T. Kiriyama, and K. Inoue,Res. Comm. Chem. Pathol. Pharmacol. 47, 203 (1985).

    CAS  Google Scholar 

  16. V. Fernandez and L. A. Videla,Experentia 37, 392 (1981).

    Article  CAS  Google Scholar 

  17. S. Morton and M. C. Mitchell,Biochem. Pharmacol. 34, 1559 (1985).

    Article  PubMed  CAS  Google Scholar 

  18. J. Vina, J. M. Estrella, C. Guerri, and F. J. Romero,Biochem. J. 188, 549 (1980).

    PubMed  CAS  Google Scholar 

  19. I. D. Capel, S. J. F. Willenbrock, M. Jenner, and D. C. Williams,Biochem. Pharmacol. 29, 1737 (1980).

    Article  PubMed  CAS  Google Scholar 

  20. A. L. Baker and A. P. Autor,Fed. Proc. 4, 2(A), 1309 (1983).

    Google Scholar 

  21. J. A. Halstead and J. C. Smith,Gastroenterology 67, 193 (1974).

    Google Scholar 

  22. A. S. Prasad,Ann. Rev. Toxicol. 20, 393 (1979).

    Article  Google Scholar 

  23. A. Flynn, S. S. Martier, R. J. Sokol, N. L. Golden, and B. C. Del Villano,Lancet 1, 572 (1981).

    Article  PubMed  CAS  Google Scholar 

  24. L. D. Keppen, T. Pysher, and O. M. Rennert,Pediat. Res. 19, 944 (1985).

    Article  PubMed  CAS  Google Scholar 

  25. T. Bergland,Zinc-Biological Effects, University of Stockholm Institute of Physics Report 84-12, University of Stockholm, Stockholm, 1984, p. 235.

    Google Scholar 

  26. I. E. Dreosti and I. R. Record,Br. J. Nutr. 41, 399 (1979).

    Article  PubMed  CAS  Google Scholar 

  27. I. E. Dreosti, S. J. Manuel, and R. A. Buckley,Br. J. Nutr. 48, 205 (1982).

    Article  PubMed  CAS  Google Scholar 

  28. T. Yoshioka, Y. Takehara, M. Shimatani, K. Abe, and K. Utsumi,Tohuku J. Exp. Med. 137, 391 (1982).

    CAS  Google Scholar 

  29. I. Mavelli, F. Autuori, L. Dini, A. Spinedi, M. R. Ciriolo, and E. Rotilio,Biochem. Biophys. Res. Comm. 102, 911 (1981).

    Article  PubMed  CAS  Google Scholar 

  30. A. K. Tanswell and B. A. Freeman,Pediatr. Res. 18, 584 (1984).

    Article  PubMed  CAS  Google Scholar 

  31. P. J. Wilkins, P. C. Grey, and I. E. Dreosti,Br. J. Nutr. 27, 113 (1972).

    Article  PubMed  CAS  Google Scholar 

  32. T. F. Slater inCompanion to Biochemistry, vol. 1, A. T. Bull, J. R. Lagnado, J. O. Thomas, and K. F. Tipton, eds., Longman, London, 1974, pp. 551–552.

    Google Scholar 

  33. E. Beutler, O. Duron, and B. M. Kelly,J. Lab. Clin. Med. 61, 882 (1963).

    PubMed  CAS  Google Scholar 

  34. A. L. Tappel inMethods in Enzymology, vol. 52, S. Fleischer and L. Packer, eds., Academic, New York, NY, 1978, pp. 506–513.

    Google Scholar 

  35. T. Asakawa and S. Matsushita,Lipids 14, 401 (1980).

    Article  Google Scholar 

  36. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall,J. Biol. Chem. 193, 265 (1951).

    PubMed  CAS  Google Scholar 

  37. N. H. Nie, C. H. Hull, J. G. Jenkins, K. Steinbrenner, and D. H. Bent,Statistical Package for the Social Sciences, McGraw-Hill, New York, NY, 1975, p. 398.

    Google Scholar 

  38. H. P. Roth and M. Kirchgessner,Zentralblatt fur Veterinarmedizin 24(A), 177 (1977).

    PubMed  CAS  Google Scholar 

  39. J. C. Wallwork, D. B. Milne, R. L. Sims, and H. H. Sandstead,J. Nutr. 113, 1895 (1983).

    PubMed  CAS  Google Scholar 

  40. E. J. Kasarskis,Exp. Neurol. 85, 114 (1984).

    Article  PubMed  CAS  Google Scholar 

  41. I. E. Dreosti,Proc. Nutr. Soc. Aust. 3, 25 (1978).

    CAS  Google Scholar 

  42. I. E. Dreosti, R. A. Buckley, and I. R. Record,Biol. Trace Elem. Res. 2, 31 (1980).

    Article  CAS  Google Scholar 

  43. G. I. Henderson, A. M. Hoyumpa, C. McClain, and S. Schenker,Alcohol Clin. Exp. Res. 3, 99 (1979).

    PubMed  CAS  Google Scholar 

  44. E. J. Kasarskis, W. I. Manton, L. D. Devenport, J. B. Kirkpatrick, G. A. Howell, M. A. Klitenick, and C. J. Frederickson,Exp. Neurol. 90, 81 (1985).

    Article  PubMed  CAS  Google Scholar 

  45. J. Wang and R. N. Pierson,J. Lab. Clin. Med. 85, 50 (1975).

    PubMed  CAS  Google Scholar 

  46. R. A. Mendelson and A. M. Huber, inCurrents in Alcoholism: Recent Advances in Research and Treatment, vol. 7, M. Galanter, ed., Grune & Stratton, New York, NY, 1980, pp. 39–48.

    Google Scholar 

  47. F. K. Ghishan, R. Patwardhan, and H. L. Greene,J. Lab. Clin. Med. 100, 45 (1982).

    PubMed  CAS  Google Scholar 

  48. A. Wendel, S. Feuerstein, and K. H. Konz, inFunction of Glutathione in Liver and Kidney, H. Sies and A. Wendel, eds., Springer-Verlag, Berlin, 1978, pp. 183–188.

    Google Scholar 

  49. R. C. Green and P. J. O'Brien,Biochem. Biophys. Acta 197, 31 (1970).

    Article  PubMed  CAS  Google Scholar 

  50. N. Oshino and B. Chance,Biochem. J. 162, 509 (1977).

    PubMed  CAS  Google Scholar 

  51. J. M. Hsu,Nutr. Rep. Int. 25, 573 (1982).

    CAS  Google Scholar 

  52. H. Fukino, M. Hirai, Y. M. Hsueh, and Y. Yamane,Toxicol. Appl. Pharmacol. 73, 395 (1984).

    Article  PubMed  CAS  Google Scholar 

  53. M. Kawata and K. T. Suzuki,Toxicol. Lett. 15, 131 (1983).

    Article  PubMed  CAS  Google Scholar 

  54. M. Chvapil, C. F. Zukoski, B. G. Hattler, L. Stankova, D. Montgomery, E. C. Carlson, and J. C. Ludwig inTrace Elements in Human Health and Disease, vol. 1, A. S. Prasad and D. Oberleas, eds., Academic, New York, NY, 1976, pp. 269–281.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dreosti, I.E., Partick, E.J. Zinc, ethanol, and lipid peroxidation in adult and fetal rats. Biol Trace Elem Res 14, 179–191 (1987). https://doi.org/10.1007/BF02795685

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02795685

Index Entries

Navigation