Advertisement

Biological Trace Element Research

, Volume 14, Issue 3, pp 179–191 | Cite as

Zinc, ethanol, and lipid peroxidation in adult and fetal rats

  • Ivor E. Dreosti
  • Eric J. Partick
Article

Abstract

Studies were performed on adult and fetal rats receiving either a zinc-deficient (<0.5 ppm) diet and/or ethanol (20%) throughout pregnancy. Liver zinc levels were depressed in fetuses exposed toin utero zinc deficiency, but brain zinc levels were unchanged. Ethanol had no effect on the concentration of zinc in the several fetal and adult tissues studies. Lipid peroxidation, as measured by endogenous levels of malondialdehyde (MDA) increased following food restriction, zinc improverishment, and alcoholism in adult and fetal livers, but not in fetal brains. Generally, levels of MDA were highest when both zinc deficiency and the ingestion of alcohol occurred concurrently. Glutathione (GSH) was depressed by zinc restriction in several adult and fetal tissues, but not in the fetal brain. Ethanol alone had no effect on GSH levels. The activity of the enzyme glutathione peroxidase (GSH-Px) was not changed in either organism by alcohol or zinc deficiency.

Overall, the data point to increased lipid peroxidation in maternal and fetal rat tissues following zinc depletion and/or treatment with alcohol and draw attention to the apparent vulnerability of the fetal liver toin utero alcoholism. By contrast, the fetal brain seems to be especially resistant to alcohol and zinc-related lipoperoxidation. An association is suggested between the increased lipoperoxidation accompanying zinc deficiency and reduced levels of GSH, but this does not appear to relate to changes in the activity of GSH-Px. A similar relationship is not evident with respect to the increased levels of MDA in fetal and adult livers following chronic alcohol intoxication. A possible basis for the zinc-GSH interaction is discussed.

Index Entries

Zinc, in adult and fetal rats ethanol, in adult and fetal rats lipid peroxidation, in adult and fetal rats malondialdehyde glutathione glutathione peroxidase zinc-glutathione peroxidase interaction, alcohol intoxication 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    M. Chvapil,Med. Clin. N. Am. 60, 799 (1967).Google Scholar
  2. 2.
    W. J. Bettger and B. L. O'Dell,Life Sci. 28, 1425 (1981).PubMedCrossRefGoogle Scholar
  3. 3.
    I. E. Dreosti, inMechanisms of Alcohol Damage in Utero, Ciba Symposium 105, Pittman, London, 1984, pp. 103–115.Google Scholar
  4. 4.
    J. F. Sullivan, M. M. Jetton, H. K. Hahn, and R. E. Burch,Am. J. Clin. Nutr. 33, 51 (1980).PubMedGoogle Scholar
  5. 5.
    I. E. Dreosti, I. R. Record, and S. J. Manuel,Biol. Trace Elem. Res. 7, 103 (1985).CrossRefGoogle Scholar
  6. 6.
    A. Valenzuela, V. Fernandez, and L. A. Videla,Toxicol. Appl. Pharmacol. 70, 87 (1983).PubMedCrossRefGoogle Scholar
  7. 7.
    L. A. Videla and A. Valenzuela,Life Sci. 31, 2395 (1982).PubMedCrossRefGoogle Scholar
  8. 8.
    R. E. Litov, D. H. Irvin, J. E. Downey, and A. L. Tappel,Lipids 13, 305 (1978).PubMedCrossRefGoogle Scholar
  9. 9.
    S. Shaw, E. Yayatilleke, W. A. Ross, E. R. Gordon, and C. Lieber,J. Lab. Clin. Med. 98, 417 (1981).PubMedGoogle Scholar
  10. 10.
    M. V. Torrielli, L. Gabriel, and M. U. Dianzani,J. Pathol. 126, 11 (1978).PubMedCrossRefGoogle Scholar
  11. 11.
    H. Speisky, D. Bunout, H. Orrego, H. G. Giles, A. Gunasekara, and Y. Israel,Res. Comm. Chem. Pathol. Pharmacol. 48, 77 (1985).Google Scholar
  12. 12.
    H. W. Sippel,Acta Pharmacol. Toxicol. 53, 135 (1983).CrossRefGoogle Scholar
  13. 13.
    C. Guerri and S. Grisolia,Pharmacol. Biochem. Behav. 13, 53 (1980).PubMedGoogle Scholar
  14. 14.
    H. Speisky, A. Macdonald, G. Giles, H. Orrego, and Y. Israel,Biochem. J. 225, 565 (1985).PubMedGoogle Scholar
  15. 15.
    Y. Kera, S. Komura, Y. Ohbora, T. Kiriyama, and K. Inoue,Res. Comm. Chem. Pathol. Pharmacol. 47, 203 (1985).Google Scholar
  16. 16.
    V. Fernandez and L. A. Videla,Experentia 37, 392 (1981).CrossRefGoogle Scholar
  17. 17.
    S. Morton and M. C. Mitchell,Biochem. Pharmacol. 34, 1559 (1985).PubMedCrossRefGoogle Scholar
  18. 18.
    J. Vina, J. M. Estrella, C. Guerri, and F. J. Romero,Biochem. J. 188, 549 (1980).PubMedGoogle Scholar
  19. 19.
    I. D. Capel, S. J. F. Willenbrock, M. Jenner, and D. C. Williams,Biochem. Pharmacol. 29, 1737 (1980).PubMedCrossRefGoogle Scholar
  20. 20.
    A. L. Baker and A. P. Autor,Fed. Proc. 4, 2(A), 1309 (1983).Google Scholar
  21. 21.
    J. A. Halstead and J. C. Smith,Gastroenterology 67, 193 (1974).Google Scholar
  22. 22.
    A. S. Prasad,Ann. Rev. Toxicol. 20, 393 (1979).CrossRefGoogle Scholar
  23. 23.
    A. Flynn, S. S. Martier, R. J. Sokol, N. L. Golden, and B. C. Del Villano,Lancet 1, 572 (1981).PubMedCrossRefGoogle Scholar
  24. 24.
    L. D. Keppen, T. Pysher, and O. M. Rennert,Pediat. Res. 19, 944 (1985).PubMedCrossRefGoogle Scholar
  25. 25.
    T. Bergland,Zinc-Biological Effects, University of Stockholm Institute of Physics Report 84-12, University of Stockholm, Stockholm, 1984, p. 235.Google Scholar
  26. 26.
    I. E. Dreosti and I. R. Record,Br. J. Nutr. 41, 399 (1979).PubMedCrossRefGoogle Scholar
  27. 27.
    I. E. Dreosti, S. J. Manuel, and R. A. Buckley,Br. J. Nutr. 48, 205 (1982).PubMedCrossRefGoogle Scholar
  28. 28.
    T. Yoshioka, Y. Takehara, M. Shimatani, K. Abe, and K. Utsumi,Tohuku J. Exp. Med. 137, 391 (1982).Google Scholar
  29. 29.
    I. Mavelli, F. Autuori, L. Dini, A. Spinedi, M. R. Ciriolo, and E. Rotilio,Biochem. Biophys. Res. Comm. 102, 911 (1981).PubMedCrossRefGoogle Scholar
  30. 30.
    A. K. Tanswell and B. A. Freeman,Pediatr. Res. 18, 584 (1984).PubMedCrossRefGoogle Scholar
  31. 31.
    P. J. Wilkins, P. C. Grey, and I. E. Dreosti,Br. J. Nutr. 27, 113 (1972).PubMedCrossRefGoogle Scholar
  32. 32.
    T. F. Slater inCompanion to Biochemistry, vol. 1, A. T. Bull, J. R. Lagnado, J. O. Thomas, and K. F. Tipton, eds., Longman, London, 1974, pp. 551–552.Google Scholar
  33. 33.
    E. Beutler, O. Duron, and B. M. Kelly,J. Lab. Clin. Med. 61, 882 (1963).PubMedGoogle Scholar
  34. 34.
    A. L. Tappel inMethods in Enzymology, vol. 52, S. Fleischer and L. Packer, eds., Academic, New York, NY, 1978, pp. 506–513.Google Scholar
  35. 35.
    T. Asakawa and S. Matsushita,Lipids 14, 401 (1980).CrossRefGoogle Scholar
  36. 36.
    O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall,J. Biol. Chem. 193, 265 (1951).PubMedGoogle Scholar
  37. 37.
    N. H. Nie, C. H. Hull, J. G. Jenkins, K. Steinbrenner, and D. H. Bent,Statistical Package for the Social Sciences, McGraw-Hill, New York, NY, 1975, p. 398.Google Scholar
  38. 38.
    H. P. Roth and M. Kirchgessner,Zentralblatt fur Veterinarmedizin 24(A), 177 (1977).PubMedGoogle Scholar
  39. 39.
    J. C. Wallwork, D. B. Milne, R. L. Sims, and H. H. Sandstead,J. Nutr. 113, 1895 (1983).PubMedGoogle Scholar
  40. 40.
    E. J. Kasarskis,Exp. Neurol. 85, 114 (1984).PubMedCrossRefGoogle Scholar
  41. 41.
    I. E. Dreosti,Proc. Nutr. Soc. Aust. 3, 25 (1978).Google Scholar
  42. 42.
    I. E. Dreosti, R. A. Buckley, and I. R. Record,Biol. Trace Elem. Res. 2, 31 (1980).CrossRefGoogle Scholar
  43. 43.
    G. I. Henderson, A. M. Hoyumpa, C. McClain, and S. Schenker,Alcohol Clin. Exp. Res. 3, 99 (1979).PubMedGoogle Scholar
  44. 44.
    E. J. Kasarskis, W. I. Manton, L. D. Devenport, J. B. Kirkpatrick, G. A. Howell, M. A. Klitenick, and C. J. Frederickson,Exp. Neurol. 90, 81 (1985).PubMedCrossRefGoogle Scholar
  45. 45.
    J. Wang and R. N. Pierson,J. Lab. Clin. Med. 85, 50 (1975).PubMedGoogle Scholar
  46. 46.
    R. A. Mendelson and A. M. Huber, inCurrents in Alcoholism: Recent Advances in Research and Treatment, vol. 7, M. Galanter, ed., Grune & Stratton, New York, NY, 1980, pp. 39–48.Google Scholar
  47. 47.
    F. K. Ghishan, R. Patwardhan, and H. L. Greene,J. Lab. Clin. Med. 100, 45 (1982).PubMedGoogle Scholar
  48. 48.
    A. Wendel, S. Feuerstein, and K. H. Konz, inFunction of Glutathione in Liver and Kidney, H. Sies and A. Wendel, eds., Springer-Verlag, Berlin, 1978, pp. 183–188.Google Scholar
  49. 49.
    R. C. Green and P. J. O'Brien,Biochem. Biophys. Acta 197, 31 (1970).PubMedCrossRefGoogle Scholar
  50. 50.
    N. Oshino and B. Chance,Biochem. J. 162, 509 (1977).PubMedGoogle Scholar
  51. 51.
    J. M. Hsu,Nutr. Rep. Int. 25, 573 (1982).Google Scholar
  52. 52.
    H. Fukino, M. Hirai, Y. M. Hsueh, and Y. Yamane,Toxicol. Appl. Pharmacol. 73, 395 (1984).PubMedCrossRefGoogle Scholar
  53. 53.
    M. Kawata and K. T. Suzuki,Toxicol. Lett. 15, 131 (1983).PubMedCrossRefGoogle Scholar
  54. 54.
    M. Chvapil, C. F. Zukoski, B. G. Hattler, L. Stankova, D. Montgomery, E. C. Carlson, and J. C. Ludwig inTrace Elements in Human Health and Disease, vol. 1, A. S. Prasad and D. Oberleas, eds., Academic, New York, NY, 1976, pp. 269–281.Google Scholar

Copyright information

© The Humana Press Inc 1987

Authors and Affiliations

  • Ivor E. Dreosti
    • 1
  • Eric J. Partick
    • 1
  1. 1.Division of Human NutritionCSIRO, AustraliaAdelaide

Personalised recommendations