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Trace elements in human parotid saliva

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Abstract

Although various proteins and some electrolytes have been measured in human saliva, little systematic data about the major and minor elemental components of this body fluid have been obtained. In order to obtain such data, concentrations of C, Na, P, Cl, K, Ca, Sc, Cr, Fe, Co, Zn, Se, Br, Rb, Sb, I, and Cs in human parotid saliva were measured by instrumental nuclear methods. The data obtained confirmed the relative lack of Zn in saliva of patients with hypogeusia (decreased taste acuity) and suggested that concentrations of Na, Cl, Br, and Ca followed the order: normals > hypogeusia > hyposmia (decreased smell acuity). To compare concentrations of elements in saliva with those in blood and urine, absolute concentrations were normalized to that of Na through the use of a concept called an enrichment factor. On this basis, parotid saliva is relatively depleted in Se, Zn, and Fe and enriched for most other elements relative to blood plasma indicating that the fluid is not simply a transudate of blood plasma. Using this same technique, saliva composition was found more similar to urine than blood plasma, being relatively depleted in Se, Cs, and Co, being enriched in I, Br, and Cr and having about the same relative concentrations of P, Cl, Zn, Fe, Ca, K, and Rb. As the total body concentrations of many of the enriched elements in saliva are extremely small, their enrichment in saliva suggests special roles for these elements in the oral cavity. Because of its accessibility, ease of collection, and interaction with some body constituents, saliva represents a useful, albeit neglected, tool in the diagnosis of some physiological and pathological changes in body function and in understanding important aspects of trace metal metabolism.

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References

  1. I. L. Shannon,IRCS Medical Sciences: Alimentary System; Biochemistry; Clinical Biochemistry; Dentistry and Oral Biology; Physiology 9, 90 (1980).

    Google Scholar 

  2. I. D. Mandel,CRC Critical Review in Clinical Laboratory Sciences, CRC Press, Boca Raton, FL, (1980).

    Google Scholar 

  3. I. Kleinberg, S. A. Ellison, and I. D. Mandel, eds.,Saliva and Dental Caries, Information Retrieval Inc., NY, (1979).

    Google Scholar 

  4. W. S. Cherwick, H. J. Eichel, and G. J. Barbero,J. Pediat. 65, 694 (1964).

    Article  Google Scholar 

  5. F. Reynolds, N. F. Jones, P. H. Ziroyanis, and S. W. Smith,Lancet II, 384 (1976).

    Article  Google Scholar 

  6. C. Bass,Lancet II, 368 (1979).

    Google Scholar 

  7. J. E. Groopman, S. Z. Salahuddin, M. G. Sarngadharan, P. D. Markham, M. Gonda, A. Suski, and R. C. Gallo,Science 226, 447 (1984).

    Article  PubMed  CAS  Google Scholar 

  8. P. Bernfeld,Meth. Enzymol. 1, 154 (1955).

    Google Scholar 

  9. R. I. Henkin, R. E. Lippoldt, J. Bilstad, and H. Edelhoch,Proc. Natl. Acad. Sci. USA 72, 488 (1975).

    Article  PubMed  CAS  Google Scholar 

  10. R. I. Henkin, R. E. Lippoldt, J. Bilstad, R. O. Wolf, C. K. L. Lum, and H. Edelhoch,J. Biol. Chem. 253, 7556 (1978).

    PubMed  CAS  Google Scholar 

  11. A. R. Shatzman and R. I. Henkin,Biochem. Med. 29, 182 (1983).

    Article  PubMed  CAS  Google Scholar 

  12. R. I. Henkin,Zinc and Copper in Clinical Medicine, K. M. Hambidge and B. L. Nichols, eds., Spectrum, Jamaica, NY, 1978, pp. 35–48.

    Google Scholar 

  13. D. Orstavik and F. W. Kraus,J. Oral. Path.,2, 68 (1973).

    Article  PubMed  CAS  Google Scholar 

  14. G. Rolla, J. E. Ciardi, and W. H. Bowen,Scand. J. Dent. Res. 91, 186 (1983).

    PubMed  CAS  Google Scholar 

  15. D. I. Hay,Cardiology Today, Karger, Basel, 1984, pp. 98–108.

    Google Scholar 

  16. R. I. Henkin, N. Talal, A. L. Larson, and C. F. T. Mattern,Ann. Int. Med. 76, 375 (1972).

    PubMed  CAS  Google Scholar 

  17. G. Bonanni and F. Perazzi,Nuntius Radiologicus 31, 383 (1965).

    PubMed  CAS  Google Scholar 

  18. A. Conger,Radiat. Res. 53, 338 (1973).

    Article  PubMed  CAS  Google Scholar 

  19. K. L. Mossman and R. I. Henkin,Int. J. Rad. Oncol. Biol. Phys. 4, 663 (1978).

    CAS  Google Scholar 

  20. A. D. Landman, L. M. Sanford, B. E. Howland, C. Dawes, and E. T. Pritchard,Experientia 32, 940 (1976).

    Article  PubMed  CAS  Google Scholar 

  21. A. M. Lawrence, S. Tam, S. Hojvat, and L. Kirsteins,Science 195, 70 (1977).

    Article  PubMed  CAS  Google Scholar 

  22. G. Carpenter and S. Cohen,Ann. Rev. Biochem. 48, 193 (1979).

    Article  PubMed  CAS  Google Scholar 

  23. R. A. Bradshaw,Ann. Rev. Biochem. 47, 191 (1978).

    Article  PubMed  CAS  Google Scholar 

  24. J. S. Law, K. Watanabe, and R. I. Henkin,Clin. Res. 32, 544A (1984).

    Google Scholar 

  25. J. S. Law and R. I. Henkin,Biochem. Med. Met. Biol. 36, 118 (1986).

    Article  CAS  Google Scholar 

  26. R. I. Henkin, P. J. Schechter, R. C. Hoye, and C. F. T. Mattern,J. Amer. Med. Assoc. 217, 434 (1971).

    Article  CAS  Google Scholar 

  27. A. R. Shatzman and R. I. Henkin,Proc. Nat. Acad. Sci. USA,78, 3867 (1981).

    Article  PubMed  CAS  Google Scholar 

  28. R. I. Henkin, C. W. Mueller, and R. O. Wolf,J. Lab. Clin. Med. 86, 175 (1975).

    PubMed  CAS  Google Scholar 

  29. R. I. Henkin, P. J. Schechter, W. T. Friedewald, D. L. DeMets, and M. S. Raff,Amer. J. Med. Sci. 272, 285 (1976).

    Article  PubMed  CAS  Google Scholar 

  30. M. S. Germani, I. Gokmen, A. C. Sigler, G. S. Kowalczyk, I. Olmez, A. M. Small, D. L. Anderson, M. Failey, M. C. Gulovali, C. E. Choquette, E. A. Lepel, G. E. Gordon, and W. H. Zoller,Anal. Chem. 52, 240 (1980).

    Article  CAS  Google Scholar 

  31. N. K. Aras, W. H. Zoller, G. E. Gordon, and G. J. Lutz,Anal. Chem. 45, 1481 (1973).

    Article  PubMed  CAS  Google Scholar 

  32. I. Olmez, N. K. Aras, G. E. Gordon, and W. H. Zoller,Anal. Chem. 46, 935 (1974).

    Article  PubMed  CAS  Google Scholar 

  33. I. Olmez, M. C. Gulovali, G. E. Gordon, and R. I. Henkin,Trace Substances in Environmental Health, vol. XII, D. D. Hemphill, ed., Univ. of Missouri, Columbia, MO, 1978, p. 231.

    Google Scholar 

  34. R. I. Henkin, R. L. Aamolt, A. K. Babcock, R. P. Agarwal and A. R. Shatzman,Perception of Behavioral Chemicals, D. M. Norris, ed., Elsevier/North Holland, Amsterdam, 1981, p. 251.

    Google Scholar 

  35. O. Levander, B. Sutherland, V. C. Morris, J. C. King,Selenium in Biology and Medicine, J. R. Spallholz, J. L. Martin, H. E. Ganther, eds., AVI Press, Westport, CT, 1981, p. 256.

    Google Scholar 

  36. O. Levander and V. C. Morris,Amer. J. Clin. Nutr. 39, 809 (1984).

    PubMed  CAS  Google Scholar 

  37. R. P. Agarwal and R. I. Henkin,Metabolism 33, 797 (1984).

    Article  PubMed  CAS  Google Scholar 

  38. R. P. Agarwal and R. I. Henkin,J. Biol. Chem. 262, 2568 (1987).

    PubMed  CAS  Google Scholar 

  39. G. V. Iyengar, W. E. Kollmer, H. J. M. Bowen,Elemental Composition of Human Tissues and Body Fluids, H. F. Ebel, ed., Verlag Chemie, Weinheim, FDR, 1978.

    Google Scholar 

  40. J. Versieck, J. Hoste, F. Barbier, H. Michels, and J. DeRudder,Clin. Chem. 23, 1301 (1977).

    PubMed  CAS  Google Scholar 

  41. J. Versieck, J. Hoste, F. Barbier, H. Sleyaert, J. DeRudder, and H. Michels, Clin. Chem.24, 303 (1978).

    PubMed  CAS  Google Scholar 

  42. R. A. Anderson, M. A. Bryden, and M. M. Polansky,Amer. J. Clin. Nutr. 41, 571 (1985).

    PubMed  CAS  Google Scholar 

  43. R. A. Anderson, M. M. Polansky, M. A. Bryden, K. Patterson, C. Veillon, and W. H. Glinsman,J. Nutr. 113, 276 (1983).

    PubMed  CAS  Google Scholar 

  44. J. M. Rosner, J. C. Macome, and D. P. Cardinali,Endocrinol. 85, 1000 (1969).

    Article  CAS  Google Scholar 

  45. Y. Ito,Acad. Sci. 85, 228 (1960).

    Article  CAS  Google Scholar 

  46. D. H. Schlessinger and D. Hay,J. Biol. Chem. 252, 1689 (1977).

    Google Scholar 

  47. H. S. Belford, E. G. Triffleman, G. D. Offner, R. F. Troxler, and F. G. Oppenheim,J. Biol. Chem. 259, 3977 (1984).

    PubMed  CAS  Google Scholar 

  48. R. E. Troxler, H. S. Belford, and F. G. Oppenheim,Biochem. Biophys. Res. Comm. 111, 239 (1983).

    Article  PubMed  CAS  Google Scholar 

  49. A. L. Weiner, P. Ofner, and E. A. Sweeney,Endocrinol. 87, 406 (1970).

    CAS  Google Scholar 

  50. J. A. Mangos,J. Dent. Res. 57, 826 (1978).

    PubMed  CAS  Google Scholar 

  51. H. Holzgreve, J. R. Martinez, and A. Vogel,Pfluegers Arch. 290, 134 (1966).

    Article  CAS  Google Scholar 

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Author notes

  1. Ilhan Olmez

    Present address: Nuclear Reactor Laboratory, Massachusetts Institute of Technology, 02139, Cambridge, MA

  2. M. Cetin Gulovali

    Present address: Turkish Aerospace Industries, Inc., Ankara, Turkey

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of Maryland, 20742, College Park, MD

    Ilhan Olmez, M. Cetin Gulovali & Glen E. Gordon

  2. Center for Molecular Nutrition and Sensory Disorders, Taste and Smell Clinic, 20016, Washington, DC

    Robert I. Henkin

Authors
  1. Ilhan Olmez
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  2. M. Cetin Gulovali
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  3. Glen E. Gordon
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  4. Robert I. Henkin
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Olmez, I., Gulovali, M.C., Gordon, G.E. et al. Trace elements in human parotid saliva. Biol Trace Elem Res 17, 259–270 (1988). https://doi.org/10.1007/BF02795462

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  • DOI: https://doi.org/10.1007/BF02795462

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