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An enzyme-linked immunoadsorbent assay for rat ceruloplasmin

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Abstract

A noncompetitive, colorimetric enzyme-linked immunoadsorbent assay (ELISA)† was developed for measuring rat ceruloplasmin in serum and in medium from culture hepatocytes. The assay utilized polystyrene immobilized antibody which bound ceruloplasmin which then bound biotinylated antibody. The biotinylated antibody-antigen complex was detected with strepavidin-alkaline phosphatase conjugate. Standard curves for rat ceruloplasmin were constructed in the range between 10 and 50 ng/mL. Increases of 10 ng produced an increase inA 403 of more than 0.2. With this immunoassay, serum ceruloplasmin levels were found to average 35 mg Cp/dL in control rats and 87 mg/dL in rats with experimental inflammation. Liver parenchymal cells secreted 1.6μg Cp/5×105 cells/24h. This ELISA assay for ceruloplasmin will facilitate studies on the regulation of ceruloplasmin synthesis and secretion in both intact rats and isolated hepatocytes.

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Abbreviations

ELISA:

enzyme linked immunoadsorbent assay

Cp :

ceruloplasmin

PBS:

phosphate buffered saline

BSA:

bovine serum albumin

and WME:

Williams minimal essential media

References

  1. R. J. Cousins,Physiol. Rev. 65, 238 (1985).

    PubMed  CAS  Google Scholar 

  2. K. S. Raju, G. Alessandri, M. Ziche, and P. M. Gullino,JNCI 69, 1183 (1982).

    PubMed  CAS  Google Scholar 

  3. M. C. Linder, J. R. Moor, and K. Wright,JNCI 67, 263 (1981).

    PubMed  CAS  Google Scholar 

  4. C. A. Owen,Biol. Trace Elem. Res. 3, 217 (1981).

    Article  CAS  Google Scholar 

  5. L. V. Puchkova, V. S. Gaitskhoki, N. K. Monakohov, L. T. Timchenko, and S. A. Neifakh,Molec. Cell Biochem. 35, 159 (1981).

    Article  PubMed  CAS  Google Scholar 

  6. K. Sugiyama, T. Emori, and S. Nagase,J. Biochem. 92, 775 (1982).

    PubMed  CAS  Google Scholar 

  7. A. L. Weiner, and R. J. Cousins,Biochem. J. 173, 486 (1983).

    CAS  Google Scholar 

  8. W. E. Rice,Anal. Biochem. 3, 452 (1962).

    Article  PubMed  CAS  Google Scholar 

  9. R. A. DiSilvestro and E. A. David,Clin. Chim. Acta 158, 287 (1986).

    Article  PubMed  CAS  Google Scholar 

  10. O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall,J. Biol. Chem. 193, 265 (1951).

    PubMed  CAS  Google Scholar 

  11. J. Goding,J. Immunological Methods 20, 241 (1978).

    Article  CAS  Google Scholar 

  12. M. N. Berry and D. S. Friend,J. Cell Biol. 43, 506 (1969).

    Article  PubMed  CAS  Google Scholar 

  13. R. A. DiSilvestro,Proc. Soc. Exp. Med. 183, 257 (1986).

    CAS  Google Scholar 

  14. N. A. Holtzman and B. M. Guamnitz,J. Biol. Chem. 245, 2345 (1970).

    Google Scholar 

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Authors and Affiliations

  1. Department of Foods and Nutrition, Purdue University, 47907, W. Lafayette, IN

    Robert A. DiSilvestro & Elizabeth A. David

  2. Department of Food Science and Human Nutrition, University of Florida, 32611, Gainesville, FL

    Ellen F. Barber & Robert J. Cousins

Authors
  1. Robert A. DiSilvestro
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  2. Ellen F. Barber
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  3. Elizabeth A. David
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  4. Robert J. Cousins
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DiSilvestro, R.A., Barber, E.F., David, E.A. et al. An enzyme-linked immunoadsorbent assay for rat ceruloplasmin. Biol Trace Elem Res 17, 1–9 (1988). https://doi.org/10.1007/BF02795442

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  • DOI: https://doi.org/10.1007/BF02795442

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