Abstract
The effects of catechin, a well-known in vitro antioxidant, on 3T3 Swiss fibroblasts are studied under different conditions of oxidative stress leading to cell proliferation or cytotoxicity. Various levels of reactive oxygen species (ROS), generated extracellularly by the xanthine-xanthine oxidase (X-XO) system, are at the origin of the biphasic effect on DNA synthesis by 3T3 Swiss fibroblasts. The addition of 10−2 U XO/mL, in the absence of exogenous X, catalyzes the production of low levels of\(O_2 ^{\dot - } \) and H2O2 in the extracellular medium, which stimulate DNA synthesis and cell division. The increase in the level of ROS, by addition of increasing X concentrations, did not enhance this effect proportionally. On the contrary, high levels of ROS inhibit DNA synthesis, the cytotoxicity induced being proportional to the level of H2O2 generated by the enzyme system. Catechin does not significantly modify DNA synthesis induced by low levels of ROS, but protects in a dose-dependent manner against the cytoxicity of high levels of ROS.
Similar content being viewed by others
References
N. Darmon, Y. Fernandez, A. Periquet, and S. Mitjavila,Free Radic. Res. Commun. 17, 97 (1992).
A. I. Huguet, S. Mañez, and M. J. AlcarazZ. Naturforsch. 45 c, 19 (1990).
J. M. Ricardo da Silva, N. Darmon, Y. Fernandez, and S. Mitjavila,J. Agr. Food Chem. 39, 1549 (1991).
S. Rafat Husain, J. Cillard, and P. Cillard,Phytochemistry. 26, 2491 (1987).
A. K. Ratty, and N. P. Das,Biochem. Med. Metab. B. 36, 69 (1988).
B. Halliwell and J. M. C. Gutteridge,Free Radical in Biology and Medicine. Clarendon, Oxford, p. 416 (1989).
G. Witz, B. D. Goldstein, M. A. Amoruso, D. Stone, and W. Troll,Proc. Am. Assoc. Cancer. Res. 21, 112 (1980).
R. M. Burger, J. Peisach, and S. B. Morwitz,Life Sci. 28, 715 (1981).
G. A. C. Murrell, M. J. O. Francis, and L. Bromley,Biochem. J. 265, 659 (1990).
B. H. Burdon, and C. Rice-Evans,Free Radic. Res. Commun. 6, 345 (1989).
F. Stirpe, T. Higgins, P. L. Tazzari, E. Rozengurt,Exp. Cell Res. 192, 635 (1991).
M. Shibanuma, T. Kuroki, and K. Nose,Oncogene. 3, 17 (1988).
D. Crawford, I. Zbinden, P. Amstad, and P. Cerutti,Oncogene. 3, 27 (1988).
E. W. Kellogg and I. Fridovich,J. Biol. Chem. 250, 8812 (1975).
A. C. Mello Filho, and R. Meneghini,Biochim. Biophys. Acta 781, 56 (1984).
J. E. Puzas, and J. S. Brand,Calcified Tissue Int. 39, 104 (1986).
M. S. Noel-Hudson, C. De Belilovsky, N. Petit, A. Lindenbaum, and J. Wepierre,Toxicol. In Vitro. 3, 103 (1989).
L. Le Goff, D. Lapeyrade, A. Bossi, M. S. Noel-Hudson, J. Bonaly, and J. Wepierre,Toxicol. In Vitro 6, 423 (1992).
M. J. Richard, P. Guiraud, A. M. Monjo, and A. Favier,Free Radic. Res. Commun. 16, 303 (1992).
E. V. Link, and P. A. Riley,Biochem. J. 249, 391 (1988).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Subirade, I., Fernandez, Y., Periquet, A. et al. Catechin protection of 3T3 Swiss fibroblasts in culture under oxidative stress. Biol Trace Elem Res 47, 313–319 (1995). https://doi.org/10.1007/BF02790132
Issue Date:
DOI: https://doi.org/10.1007/BF02790132