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Increase in cellular pool of low-molecular-weight iron during ethanol metabolism in rat hepatocyte cultures

Relationship with lipid peroxidation

  • Part VI Renal. Liver, and Inflammatory Disorders
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Abstract

Ethanol-induced lipid peroxidation was studied in primary rat hepatocyte cultures supplemented with ethanol at the concentration of 50 mM. Lipid peroxidation was assessed by two indices: (1) conjugated dienes by second-derivative UV spectroscopy in lipid extract of hepatocytes (intracellular content), and (2) free malondialdehyde (MDA) by HPLC-UV detection and quantitation for the incubation medium (extracellular content). In cultures supplemented with ethanol, free MDA increased significantly in culture media, whereas no elevation of conjugated diene level was observed in the corresponding hepatocytes. The cellular pool of low-mol-wt (LMW) iron was also evaluated in the hepatocytes using an electron spin resonance procedure. An early increase of intracellular LMW iron (≤1 hr) was observed in ethanol-supplemented cultures; it was inhibited by 4-methylpyrazole, an inhibitor of alcohol dehydrogenase, whereas α-tocopherol, which prevented lipid peroxidation, did not inhibit the increase of LMW iron. Therefore, the LMW iron elevation was the result of ethanol metabolism and was not secondarily induced by lipid hydroperoxides. Thus, ethanol caused lipid peroxidation in rat hepatocytes as shown by the increase of free MDA, although no conjugated diene elevation was detected. During ethanol metabolism, an increase in cellular LMW iron was observed that could enhance conjugated diene degradation.

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References

  1. N. R. Di Luzio and A. D. Hartman,Fed. Proc. 26, 1436–1442 (1967).

    PubMed  Google Scholar 

  2. R. C. Reitz,Biochim. Biophys. Acta 380, 145–154 (1975).

    PubMed  CAS  Google Scholar 

  3. J. Harata, M. Nagata, E. Sasaki, I. Ishiguro, Y. Ohta, and Y. Murukami,Biochem. Pharmacol. 32, 1795–1798 (1983).

    Article  PubMed  CAS  Google Scholar 

  4. T. Kawase, S. Kato, and C. S. Lieber,Hepatology 10, 815–821 (1989).

    Article  PubMed  CAS  Google Scholar 

  5. T. Castillo, D. R. Koop, S. Kamimura, G. Triadafilopoulos, and H. Tsukamoto,Hepatology 16, 992–996 (1992).

    Article  PubMed  CAS  Google Scholar 

  6. S. Hashimoto, and R. O. Recknagel,Exp. Mol. Pathol. 8, 225–242 (1968).

    Article  PubMed  CAS  Google Scholar 

  7. R. Scheig and G. Klatskin,Life Sci. 8, 855–865 (1969).

    Article  PubMed  CAS  Google Scholar 

  8. A. M. B. Reid and T. F. Slater,Biochem. Soc. Trans. 5, 1292–1296 (1977).

    PubMed  CAS  Google Scholar 

  9. H. Speisky, D. Bunout, H. Orrego, H. G. Giles, A. Gunasekara, and Y. IsraelRes. Commun. Chem. Pathol. Pharmacol. 48, 77–90 (1985).

    PubMed  CAS  Google Scholar 

  10. T. Inomata, G. A. Rao, and H. Tsukamoto,Liver 7, 233–239 (1987).

    PubMed  CAS  Google Scholar 

  11. T. E. Stege,Res. Commun. Chem. Pathol. Pharmacol. 36, 287–292 (1982).

    PubMed  CAS  Google Scholar 

  12. A. Müller and H. Sies,Biochem. J. 206, 153–156 (1982).

    PubMed  Google Scholar 

  13. S. Shaw and E. Jayatilleke,Biochem. Biophys. Res. Commun. 143, 984–990 (1987).

    Article  PubMed  CAS  Google Scholar 

  14. G. Ekströom, and M. Ingelman-Sündberg,Biochem. Pharmacol. 38, 1313–1319 (1989).

    Article  CAS  Google Scholar 

  15. S. Shaw and E. Jayatilleke,Biochem. J. 268, 579–583 (1990).

    PubMed  CAS  Google Scholar 

  16. F. R. Weiner, M. J. Czaja, and M. A. Zern,The Liver: Biology and Pathobiology, 2nd ed., I. M. Arias, W. B. Jakoby, H. Popper, D. Schachter, and D. A. Shafritz, eds., Raven, New York, pp. 1169–1193 (1988).

    Google Scholar 

  17. C. Guguen, A. Guillouzo, M. Boisnard, A. Le Cam, and M. Bourel,Biol. Gastroenterol. 8, 223–231 (1975).

    CAS  Google Scholar 

  18. L. Folch, M. Lees, and G. H. Sloane-Stanley,J. Biol. Chem. 226, 497–509 (1957).

    PubMed  CAS  Google Scholar 

  19. F. P. Corongiu, G. Poli, M. U. Dianzani, K. H. Cheeseman, and T. F. Slater,Chem. Biol. Interact. 59, 147–155 (1986).

    Article  PubMed  CAS  Google Scholar 

  20. O. Sergent, I. Morel, P. Cogrel, M. Chevanne, M. Beaugendre, P. Cillard, and J. Cillard,Anal. Biochem. 211, 219–223 (1993).

    Article  PubMed  CAS  Google Scholar 

  21. A. S. Csallany, D. M. Guan, J. D. Manwaring, and P. B. Addis,Anal. Biochem. 142, 277–283 (1984).

    Article  PubMed  CAS  Google Scholar 

  22. A. V. Kozlov, D. Y. Yegorov, Y. A. Vladimirov, and O. A. Azizova,Free Rad. Biol. Med. 13, 9–16 (1992).

    Article  PubMed  CAS  Google Scholar 

  23. J. Bunyan, M. A. Cawthorne, A. T. Diplock, and J. Green,Br. J. Nutr. 23, 309–317 (1969).

    Article  PubMed  CAS  Google Scholar 

  24. M. V. Torrielli, L. Gabriel, and M. U. Dianzani,J. Pathol. 126, 11–25 (1978).

    Article  PubMed  CAS  Google Scholar 

  25. M. U. Dianzani and M. V. Torrielli,Medicine Biologie Environnement 9, 179–190 (1981).

    CAS  Google Scholar 

  26. V. D. Antonenkov, S. V. Pirohkov, S. V. Popova, and L. F. Panchenko,Int. J. Biochem. 21, 1191–1195 (1989).

    Article  PubMed  CAS  Google Scholar 

  27. S. D. Aust and B. A. Svingen, inFree Radicals in Biology, vol. 5, W. A. Pryor, ed., Academic, New York, pp. 1–28 (1982).

    Google Scholar 

  28. M. J. Davies, inFree Radicals, Metal Ions and Biopolymers, P. C. Beaumont, D. J. Deeble, B. J. Parsons, and C. Rice-Evans, eds., Richelieu, London, pp. 303–311 (1989).

    Google Scholar 

  29. H. Rouach, P. Houze, M-T. Orfanelli, M. Gentil, R. Bourdon, and R. Nordmann,Biochem. Pharmacol. 39, 1095–1100 (1990).

    Article  PubMed  CAS  Google Scholar 

  30. S. Shaw, E. Jayatilleke, and C. S. Lieber,Alcohol 5, 135–140 (1988).

    Article  PubMed  CAS  Google Scholar 

  31. R. Topham, M. Goger, K. Pearce, and P. Schultz,Biochem. J. 261, 137–143 (1989).

    PubMed  CAS  Google Scholar 

  32. G. Minotti,Arch. Biochem. Biophys. 273, 137–143 (1989).

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire et Végétale, INSERM U49, UFR des Sciences Pharmaceutiques, 2, Av, du Pr. Léon Bernard, 35043, Rennes Cedex, France

    O. Sergent, I. Morel, P. Cogrel, M. Chevanne, P. Cillard & J. Cillard

  2. INSERM U49, Unité de Recherches Hépatologiques, Hôpital Pontchaillou, Rennes, France

    N. Pasdeloup, P. Brissot & G. Lescoat

Authors
  1. O. Sergent
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  2. I. Morel
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  3. P. Cogrel
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  4. M. Chevanne
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  5. N. Pasdeloup
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  6. P. Brissot
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  7. G. Lescoat
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  8. P. Cillard
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  9. J. Cillard
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Sergent, O., Morel, I., Cogrel, P. et al. Increase in cellular pool of low-molecular-weight iron during ethanol metabolism in rat hepatocyte cultures. Biol Trace Elem Res 47, 185–192 (1995). https://doi.org/10.1007/BF02790116

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