Abstract
The aim of the study was to investigate the effect of zinc depletion on the susceptibility of Wistar rat low-density lipoproteins (LDL) to peroxidation and their uptake by macrophages, before and after in vitro oxidation. The rats were fed for 7 wk a Zn-adequate diet (100 ppm) ad libitum (AL), a Zn-deficient diet (0.2 ppm) ad libitum (ZD), or a Zn-adequate diet according to the pair-feeding method (PF). Zinc status was determined and, for each group, blood was pooled, and LDL were isolated and labeled with125Iodine. An aliquot of each LDL sample was oxidized using FeII 10 μM/ascorbate 250 μM. Oxidized and nonoxidized (native) LDL were incubated with P 388 D1 macrophages, and their rates of uptake and degradation by macrophages were measured. Before oxidation, LDL uptake and degradation were not modified by the diet, suggesting that Zn deficiency did not modify rat LDL in vivo. After oxidation, both LDL uptake and degradation were significantly enhanced in the three groups. Nevertheless, we did not observe a significant effect of Zn deficiency. This observation suggests that, in our experimental conditions, Zn deficiency did not modify LDL catabolism.
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References
B. Halliwell,Br. J. Exp. Pathol. 70, 737 (1989).
D. Steinberg, S. Parthasarathy, T. E. Carew, J. C. Khoo, and J. L. Witztum,New Engl. J. Med. 320, 915 (1989).
B. A. Kottke,J. Cardiovasc. Pharmacol. 10 (Suppl. 9), S7 (1987).
T. M. Bray and W. J. Bettger,Free Radic. Biol. Med. 8, 281 (1990).
T. Günther and V. Höllriegl,Biol. Trace Element Res. 22, 165, (1989).
C. Coudray, F. Boucher, M. J. Richard, J. Arnaud, J. De Leiris, and A. Favier,Biol. Trace Element Res. 30, 103 (1991).
P. Faure, A. M. Roussel, M. J. Richard, T. Foulon, P. Groslambert, A. Hadjian, and A. Favier,Biol. Trace Element Res. 28, 135 (1991).
J. Arnaud, J. Bellanger, P. Bienvenu, P. Chappuis, and A. Favier,Ann. Biol. Clin. 44, 77 (1984).
C. M. Radding and D. Steinberg,J. Clin. Invest. 39, 1560 (1960).
G. L. Peterson,Anal. Biochem. 83, 346 (1977).
P. Gambert, M. Farnier, C. Bouzerand, A. Athias, and C. Lallemant,Clin. Chim. Acta 172, 183 (1988).
D. W. Bilheimer, S. Eisenberg, and R. I. Levy,Biochim. Biophys. Acta 260, 212 (1972).
F. B. Kraemer, K. Tavangar, R. K. Gandjei, K. Kirlew, and S. R. Behr,Arteriosclerosis 10, 8 (1990).
S. C. Cunnane, C. C. Ainley, P. W. N. Keeling, R. P. H. Thompson, and M. A. Crawford,J. Am. Coll. Nutr. 5, 451 (1986).
S. C. Cunnane,Prog. Food Nutr. Sci. 12, 151 (1988).
H. Zhang, H. J. K. Basra, and U. P. Steinbrecher,J. Lipid Res. 31, 1361 (1990).
S. Koga, D. L. Horwitz, and A. M. Scanus,J. Lipid Res. 10, 577 (1969).
J. F. Nagelkerke, L. Havekes, V. W. M. Van Hinsberg, and T. J. C. Van Berkel,Arteriosclerosis 4, 256 (1984).
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Schmuck, A., Tricot, F., Hadjian, A. et al. Zinc deficiency does not enhance LDL uptake by P 388 D1 macrophages in vitro. Biol Trace Elem Res 47, 75–80 (1995). https://doi.org/10.1007/BF02790103
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DOI: https://doi.org/10.1007/BF02790103