Abstract
We investigated the effects of an OH⋅ (Fe2+/H2O2) generator system of erythrocyte membrane, particularly the time-course of lipid peroxidation as estimated by measurement of conjugated dienes, thiobarbituric reactive substances (TBARS), lipofuscin-like pigments, and α-tocopherol. Polyunsaturated fatty acids (PUFAs), especially arachidonic acid (20∶4 ω 6) and docosahexenoic acid (22∶6 ω 3), were also measured. Erythrocyte membranes were suspended in phosphate buffer containing Fe2+ (200 μM) and H2O2 (1.42 mM), and incubated in a shaking water bath at 37°C. Initially, there was an increase in TBARS and lipofuscin-like pigments, two well-known end products of PUFA oxidative degradation, whereas PUFAs remained unchanged (incubation time: 1 h). After two or more hours of incubation, marked lipid peroxidation was noted, with the appearance of conjugated dienes and a decrease of PUFAs, indicating that lipid peroxidation had occurred after a lag phase during which TBARS were not produced from PUFAs. This suggests that another OH⋅ target was involved.
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Tallineau, C., Barrier, L., Fauconneau, B. et al. Lack of correlation between TBARS production and PUFA degradation during incubation of membrane erythrocytes in an OH⋅ (Fe2+/H2O2) generator system. Biol Trace Elem Res 47, 3–7 (1995). https://doi.org/10.1007/BF02790095
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DOI: https://doi.org/10.1007/BF02790095