Summary
A simple, sensitive, and robust method has been developed for the separation and quantification of glutamate in beverages and foodstuffs. Samples were derivatized with 5 × 10−4 M fluorescein isothiocyanate. Glutamate was separated by capillary electrophoresis and detected by laser-induced fluorescence with 488-nm radiation from an argon ion laser. Response was linearly dependent on concentration between 10−7 and 10−4 M. The limit of mass detection was 16 amol and the limit of concentration detection was 5.4×10−10 M. Intra- and interday precision were 3.3%RSD (n=11) and 4.8%RSD (n=7), respectively. The method has been applied to beverages and other foods.
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Pérez-Ruiz, T., Martínez-Lozano, C., Sanz, A. et al. Analysis of glutamate in beverages and foodstuffs by capillary electrophoresis with laser-induced fluorescence detection. Chromatographia 52, 599–602 (2000). https://doi.org/10.1007/BF02789758
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DOI: https://doi.org/10.1007/BF02789758