Summary
Validation of an isocratic HPLC method for determination of methylxantines, chlorogenic acid and some hydroxy-benzoic/cinnamic acids in nervine foods and beverages is described. The method proposed can be usefully employed by single-wavelength/UV/HPLC-equipped Quality Control Laboratories. The ABZ+ Plus phase permitted complete separation of 10 compounds in 20 min. k, asymmetry of peaks, number of theoretical plates at different pH (3.0, 3.5, 4.0) and temperature (30, 40°C) were considered to determine best separation conditions. The method was then applied to some commercial samples of cocoa, coffee, tea and cola drinks, showing its real usefulness on quality control of these foods, particularly in monitoring roasting processes.
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Spiller, M.A. In:The methylxantine beverages and foods: chemistry, consumption and health effects. Alan Liss, New York,1984.
Purdon, M.P.; McCamey, D.A.J. Food Sci. 1987,52, 1680–1683.
Trugo, L.C.; Macrae, R.Food Chem. 1984,15, 219–227.
Pictet, G.; Rehacek, J. ASIC, 10e Colloque, Salvador,1982.
Amorim, H.V.; Teixeira, A.A.; Guercio, M.A.; Cruz, V.F.; Malavolta, E.Turrialba 1974,24, 217–221.
Osawa, K.; Matsumoto, T.; Maruyama, T.; Naito, Y.; Okuda, K.; Takazoe, I.Bull. Tokyo Dent. Coll. 1990,31, 125–128.
Azizah, A.H.; Ruslawati Nik, N.M.; Swee Tee, T.Food Chem. 1999, 64, 199–202.
Shahrzad, S.; Bitsch, I.J. Chromatography A 1996,16, 223–231.
Sanbongi, C.; Osakabe, N.; Netsume, M.; Takizawa, T.; Gomi, S.; Osawa, T.J. Agric. Food Chem. 1998,46, 454–457.
Yen, G.C.; Duh, P.D.J. Am. Oil Chem. Soc. 1993,70, 383–386.
Nakakuki, H.; Horie, H.; Yamauchi, Y.; Kohata, K.J. Chromatogr. A 1999,848, 523–527.
Kouno, Y.; Samizo, K.; Ishizuka, H.; Ishikura, C.Yakugaku Zasshi 1997,117, 178–184.
Schreiber-Deturmeny, E.; Bruguerolle, B.J. Chromatogr. B Biomed. Appl. 1996,677, 305–312.
Kreiser, W.R.; Martin, R.A. Jr.J. Assoc. Off. Anal. Chem. 1978,61, 1424–1427.
Tanaka, T.J. Chromatogr. A 1978,575, 311–315.
Raynal, J.; Moutounet, M.; Souquet, J.M.J. Agric. Food Chem. 1989,37, 1046–1050.
Gasco-Lopez, A.I.; Santos-Montes, A.; Izquierdo-Hornillos, R.J. Chromatogr. Sci. 1997,35, 525–535.
Reta, M.; Carr P.W.J. Chromatogr. A 1999,855, 121–127.
Liu, F.; Ascah, T.The Reporter, International Bulletin Supelco 1994,13, 2–5.
Ascah, T.; Liu F.The Reporter, International Bulletin Supelco 1995,14, 4–7.
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Arlorio, M., Coïsson, J.D. & Martelli, A. Validation of an isocratic HPLC method based on the use of ABZ+ plus phase for the simultaneous determination of methylxantines, chlorogenic acid, some hydroxy-benzoic and hydroxy-cinnamic acids. application to cocoa, coffee, tea and cola-drinks. Chromatographia 52, 579–583 (2000). https://doi.org/10.1007/BF02789754
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DOI: https://doi.org/10.1007/BF02789754