Skip to main content
Log in

The mechanism for the effect of selenium supplementation on immunity

  • Original Articles
  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

Lipid peroxide (LPO) in lymphocytes from mice was evaluated by measuring substances reactive to thiobarbituric acid (TBA). The product resulting from the reaction of TBA with lymphocytes was extracted with n-butyl and fluorescence intensity was determined. The degree of lipid peroxidation, expressed as fluorescence intensity f547, was assessed for stimulation of lymphocytes with concanavalin A (Con A), and was related to lymphocyte proliferation in response to Con A if Se was administered. The lymphocyte proliferation was determined by [3H]thymidine incorporation, expressed as cpm. The effect of superoxide dismutase (SOD), added to cell culture on lymphocyte proliferation was also evaluated. It was found that LPO in lymphocytes before Con A stimulation was significantly less than that after stimulation (p<0.001), and that SOD promoted lymphocyte proliferation dose dependently. The addition of Na2SeO3 to lymphocyte culture or supplementation in drinking water to mice decreased the produced LPO in lymphocyte in response to Con A. In the presence of Se, there is an inverse correlation between the levels of LPO in lymphocyte and the stimulated proliferation (r=−0.8902,r=−0.9439). In conclusion, active oxygen species scavenging was proposed as one of the mechanisms for Se to promote immunity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. G. N. Schrauzer, D. A. White, and C. J. Schneider,Bioinorg. Chem. 7, 23 (1977).

    Article  PubMed  CAS  Google Scholar 

  2. R. F. Burk,Ann. Rev. Nutr. 3, 53 (1983).

    Article  CAS  Google Scholar 

  3. L. C. Clark,Fed. Proc. 44, 2584 (1985).

    PubMed  CAS  Google Scholar 

  4. A. C. Griffin,Adv. Cancer Res. 29, 419 (1979).

    PubMed  CAS  Google Scholar 

  5. K. J. Helzsouer,Sem. Oncol. 10, 305 (1983).

    Google Scholar 

  6. J. T. Salonen, G. Alfthan, J. K. Huttunen, and P. Puska,Am. J. Epidemiol. 120, 34 (1984).

    Google Scholar 

  7. H. J. Thompson,J. Agric. Food Chem. 32, 422 (1984).

    Article  CAS  Google Scholar 

  8. B. M. Dworkin, W. S. Rosenthal, and G. P. Wormser,J. Paret. Ent Nutr. 10, 405 (1986).

    CAS  Google Scholar 

  9. B. M. Dworkin, W. S. Rosenthal, G. P. Wormser, L. Weiss, M. Nunez, C. Joline, and A. Herp,Biol. Trace Elem. Res. 15, 167–177 (1988).

    PubMed  CAS  Google Scholar 

  10. L. Olmsted, G. N. Schrauzer, M. Flores-Arce, and J. Dowd,Biol. Trace Elem. Res. 20, 59–65 (1989).

    Article  PubMed  CAS  Google Scholar 

  11. M. Y. Shelly, Fr. Demande, FR 2,609, 632 (C1 A61K31/485) 22 Jul. 1988, Appl. 87/670, 21 Jan., 1987.

    Google Scholar 

  12. L. Kiremidjian-Schumacher, and G. Stotzky,Environ. Res. 42, 277–903 (1987).

    Article  PubMed  CAS  Google Scholar 

  13. E. C. Segerson and D. W. Libby,Biol. Reprod. 30, 126 (1984).

    Article  PubMed  CAS  Google Scholar 

  14. Y. C. Weng, X. P. Wang, et al.Xibao Shengwuzue zazhi 7, 142–144 (1985) (Chinese).

    CAS  Google Scholar 

  15. B. F. Zhu,Guowai Yixue Mianyixue Fence,13, 140–143 (1990) (Chinese).

    Google Scholar 

  16. M. Shingu, et al.,Inflammation 11, 143 (1985).

    Article  Google Scholar 

  17. Y. Ozaki, et al.,Inflammation 10, 119 (1986).

    Article  PubMed  CAS  Google Scholar 

  18. S. Hashimoto, et al.,Infect. Immunol. 44, 66 (1984).

    Google Scholar 

  19. K. Wroemann, et al.,Eur. J. Immunol. 8, 749 (1978).

    Article  Google Scholar 

  20. B. K. Mookerjee, et al.Immunol. Commun. 9, 653 (1980).

    PubMed  CAS  Google Scholar 

  21. E. Peterhans, et al.,Eur. Immunol. 10, 477 (1980).

    Article  Google Scholar 

  22. L. S. Wong and J. L. Kiel,Immunol. Invest. 14, 503 (1980).

    Article  Google Scholar 

  23. GOC Ekejiadu, et al.,Vet. Immunol. Immunolpathol. 11, 175 (1986).

    Article  Google Scholar 

  24. X. J. Zhang, L. Liu,Zhonghua Weishengwuxue He Mianyixue Zazhi,10, 195–198 (1990). (Chinese).

    Google Scholar 

  25. X. J. Zhang, L. Liu,Shengwuhuaxue Yu Shengwuwuli Jinzhan,17, 444–447 (1990) (Chinese).

    Google Scholar 

  26. X. J. Zhang,Shengwuhuaxue Yu Shengwuwuli Jinzhan,17, 430–433 (1990) (Chinese).

    Google Scholar 

  27. D. A. Hume and M. J. Weidemann,J. Cell Physiol. 96, 306 (1978).

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sun, E., Xu, H., Liu, Q. et al. The mechanism for the effect of selenium supplementation on immunity. Biol Trace Elem Res 48, 231–238 (1995). https://doi.org/10.1007/BF02789405

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02789405

Index Entries

Navigation