Abstract
A detailed method is presented tor the creation of head-to-tail multimers of short blunt restriction fragments, ligaled into a plasmid vector in a singletube: reaction. Random priming of the concatemer insert readily yields hybridization probes of high specificity, unattainable from the short monomer fragments.
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Kapros, T., Robertson, A.J. & Waterborg, J.H. A simple method to make better probes from short DNA fragments. Mol Biotechnol 2, 95–98 (1994). https://doi.org/10.1007/BF02789291
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DOI: https://doi.org/10.1007/BF02789291