Abstract
A number of phosphodiesterases, some of which possess additional biological activities (e.g., antitumor, immunosupressive, and so on), have been considered for use in targeted tumor therapy. We propose Deoxyribonuclease I (DNase I), a compact, monomeric enzyme, as a very attractive candidate for targeting to tumor cells. Only a small amount of enzyme targeted to a cell needs to enter the nucleus in order to degrade the chromosomal DNA, making a cell incapable of further replication. We describe preliminary data on the construction of a potent single-chain antibody (scFv) immunotoxin based on bovine pancreatic DNase I. The use of a mammalian enzyme should be much less toxic and less immunogenic than current immunotoxins and may expand the current limits of immunotoxin therapy.
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Linardou, H., Deonarain, M.P., Spooner, R.A. et al. Deoxyribonuclease I (DNase I). Cell Biophysics 24, 243–248 (1994). https://doi.org/10.1007/BF02789235
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DOI: https://doi.org/10.1007/BF02789235