Abstract
The possibility for industrialization of the catalytic Multistage Fixed Bed Tower (MFBT) bioreactor using the mineral kissiris as a promoting material is examined in a large-scale pilot plant using 7000 and 100,000 L bioreactors separately. The stability of kissiris’ structure, the viability of culture, and the formation of volatile compounds were studied from batch to batch. This mineral, being a volcanic and foaming silicious rock with an average cost of $60/t, was examined by X-ray defractometer and found to mainly consist of anorthite. The stability of the catalyst not being infected was studied by an electron microscope and the viability of culture was estimated by the percentage of dead cells and the percentage of bacteria. Therefore, viability of culture was at a good level and the structure of catalyst, as related with low-residual sugar, remained constant from batch to batch, even after a preservation process of at least 6 mo. Likewise, ethyl-acetate was increased and higher alcohols were decreased in the overall period of experiments.
Similar content being viewed by others
References
Tsoutsas, T., Kanellaki, M., Psarianos, C., Kalliafas, A., and Koutinas, A. A. (1990),J. Ferment. Bioeng. 69(2), 93.
Koutinas, A. A., Kanellaki, M., Voliotis, S., Kouinis, J., Kalliafas, A., Kana, K., and Iconomou, L.Patent Cooperation Treaty (PCT), International Publication Number WO 93/01298 and International Publication Date, 21 January, 1993.
Bakoyianis, V. and Koutinas, A. A.Biotechnol. Bioeng. 1995, in press.
Kana, K., Kanellaki, M., and Koutinas, A. A. (1992),Food Biotechnol. 6, 65.
Kana, K. Doctoral dissertation, University of Patras, Patras, Greece, 1989.
Bakoyianis, V., Kanellaki, M., Kalliafas, A., and Koutinas, A. A. (1993),J. Agric. Food Chem. 41(3), 465.
Greenshieds, R. N. (1974),J. Sci. Food Agric. 25, 1307.
Kana, K., Kanellaki, M., Psarianos, C., and Koutinas, A. A. (1989),J. Ferment. Bioeng. 68(2), 144.
Cachot, T., Muller, M., and Pons, M.-N. (1991),Appl. Micribiol. Biotechnol. 35, 450.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Koutinas, A.A., Bakoyianis, V., Argiriou, T. et al. A qualitative outline to industrialize alcohol production by catalytic multistage fixed bed tower (MFBT) bioreactor. Appl Biochem Biotechnol 66, 121–131 (1997). https://doi.org/10.1007/BF02788757
Issue Date:
DOI: https://doi.org/10.1007/BF02788757