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Preparation and preliminary characterization of poly(ethylene glycol)-pepstatin conjugate

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Abstract

The carboxyl function of pepstatin has been coupled, through an amide bond, to methoxypoly(ethylene glycol) (5 kDa), to which an amino function had been previously grafted. The mPEG-pepstatin conjugate inhibits hog pepsin (aspartic proteinase) in vitro as pepstatin itself, however, with a 400 times higher apparent Ki. The conjugate apparently does not inhibit proteinases belonging to other proteinase families such as serine (trypsin, carboxypeptidase Y), cysteine (Papaya proteinase III), or metallo (collagenase) proteinases.

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References

  1. Katoh, I., Yoshinaka, Y., Rein, A., Shibuya, M., Odaka, T., and Oroszlan S. (1985),Virology 145, 280–292.

    Article  CAS  Google Scholar 

  2. Huff, J. R. (1991),J. Med. Chem. 34, 2305–2314.

    Article  CAS  Google Scholar 

  3. Tomasselli, A. G., Howe, W. J., Sawyer, T. K., Wlodawer, A., and Heinriksat, R. L. (1991),Chem. Oggi 9, 6–27.

    CAS  Google Scholar 

  4. Rich, D. H., Vara Prasad, J. V. N., Sun, C. Q., Green, J., Mueller, R., Houseman, K., MacKenzie, D., and Malkovsky, M. (1992),J. Med. Chem. 35, 3803–3812.

    Article  CAS  Google Scholar 

  5. De Camp, D. L., Babé, L. M., Salto, R., Lucich, J. L., Koo, M. S., Kahl, S. B., and Craik, C. S. (1992),J. Med. Chem. 35, 3426–3428.

    Article  Google Scholar 

  6. Mimato, T., Imai, J., Kisanuki, S., Enomoto, H., Hattori, N., Akaji, K., and Kiso, Y. (1992),Chem. Pharm. Bull. 40, 2251–2253.

    Google Scholar 

  7. Fehrentz, J. A., Chomier, B., Bignon, E., Venaud, S., Chermann, J. C., and Nisato, D. (1992),Biochem. Biophys. Res. Commun. 188, 865–872.

    Article  CAS  Google Scholar 

  8. Fehrentz, J. A., Chomier, B., Bignon, E., Venaud, S., Chermann, J. C., and Nisato, D. (1992),Biochem. Biophys. Res. Commun. 188, 873–878.

    Article  CAS  Google Scholar 

  9. Beckman, J. S., Minor, R. L., White, C. W., Repine, J. E., Rosen, G. M., and Freeman, B. A. (1988),J. Biol. Chem. 263, 6884–6892.

    CAS  Google Scholar 

  10. Caliceti, P., Schiavon, O., Mocali, R., and Veronese, F. M. (1989),II Farmaco 44, 711–720.

    CAS  Google Scholar 

  11. Matsuyama, H., Tomita, M., Taguchi, R., and Ikezawa, H. (1992),Chem. Pharm. Bull. 40, 2478–2482.

    CAS  Google Scholar 

  12. Roseng, L., Tolleshoug, H., and Berg, T. (1992),J. Biol. Chem. 267, 22,987–22,993.

    CAS  Google Scholar 

  13. Kao, K. N., Constabel, F., Michayluck, M. R., and Gamborg, O. L. (1974),Planta 120, 215–227.

    Article  CAS  Google Scholar 

  14. Ahkong, Q. F., Fischer, D., Tampion, W., and Lucy, J. A. (1975),Nature 253, 194,195.

    Article  CAS  Google Scholar 

  15. Pontecorvo, G. (1975),Somat. Cell Genet. 1, 397–400.

    Article  CAS  Google Scholar 

  16. Yamazaki, M. and Ito, T. (1990),Biochemistry 29, 1309–1314.

    Article  CAS  Google Scholar 

  17. Boni, L. T., Hah, J. S., Hui, S. W., Mukherjee, F., Ho, J. T., and Jung, C. Y. (1984),Biochem. Biophys. Acta 775, 409–418.

    Article  CAS  Google Scholar 

  18. Bittner, G. D., Ballinger, M. L., and Raymond, M. A. (1986),Brain Res. 367, 351–355.

    Article  CAS  Google Scholar 

  19. Krause, T. L. and Bittner, G. D. (1990),Proc. Natl. Acad. Sci. USA 87, 1471–1475.

    Article  CAS  Google Scholar 

  20. Geron, N. and Meiri, H. (1985),Biochim Biophys. Ada 819, 258–262.

    Article  CAS  Google Scholar 

  21. Walter, H., Raymond, F. D., and Fischer, D. (1992),J. Chromatog. 609, 219–227.

    Article  CAS  Google Scholar 

  22. Katoh, I., Yasunaga, T., Ikawa, Y., and Yoshinaka, Y. (1987),Nature 329, 654–656.

    Article  CAS  Google Scholar 

  23. Seelmeier, S., Schmidt, H., Turk, V., and Von der Helm, K. (1988),Proc. Natl. Acad. Sci. USA 85, 6612–6616.

    Article  CAS  Google Scholar 

  24. Richards, A. D., Roerts, R., Dunn, B. M., Graves, M. C., and Kay, J. (1989),FEBS Lett. 247, 113–117.

    Article  CAS  Google Scholar 

  25. Brygier, J., Gelbcke, M., Guermant, C., Nijs, M., Baeyens-Volant, D., and Looze, Y. (1993),Biotechnol. Appl. Biochem. 42, 127–136.

    Article  CAS  Google Scholar 

  26. Stocks, S. J., Jones, A. J. M., Ramey, C. W., and Brooks, D. E. (1986),Anal. Biochem. 154, 232–234.

    Article  CAS  Google Scholar 

  27. Jackson, W. T., Schlamowitz, M., and Shaw, A. (1965),Biochemistry 4, 1537–1543.

    Article  CAS  Google Scholar 

  28. Erlanger, B. F., Kokowsky, N., and Cohen, W. (1961),Arch. Biochem. Biophys. 95, 271–278.

    Article  CAS  Google Scholar 

  29. Dubois, T., Jacquet, A., Schnek, A. G., and Looze, Y. (1988),Biol. Chem. Hope-Seyler 369, 733–740.

    CAS  Google Scholar 

  30. Aibara, S., Hayashi, R., and Hata, Y. (1971),Agr. Biol. Chem. 35, 658–666.

    Google Scholar 

  31. Van Wart, H. E. and Steinbrink, D. R. (1981),Anal. Biochem. 113, 356–365.

    Article  Google Scholar 

  32. Belleau, B. and Malek, G. (1968),J. Am. Chem. Soc. 90, 1651, 1652.

    Article  CAS  Google Scholar 

  33. Cha, S. (1975),Biochem. Pharmacol. 24, 2177–2185.

    Article  CAS  Google Scholar 

  34. Morrisson, J. F. (1969),Biochim. Biophys. Acta 185, 269–286.

    Google Scholar 

  35. Workman, R. J. and Burkitt, D. W. (1979),Arch. Biochem. Biophys. 194, 157–164.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Protein Chemistry unit, Faculty of Medicine, University of Brussels, Campus Plaine, CP 206/4 Boulevard du Triomphe, B-1050, Brussels, Belgium

    Jeanne Brygier, Jean Vlncentelli, Michelle Nljs, Claude Guermant, Claudine Paul, Danielle Baeyens-Volant & Yvan Looze

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  1. Jeanne Brygier
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  2. Jean Vlncentelli
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  3. Michelle Nljs
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  4. Claude Guermant
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  5. Claudine Paul
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  6. Danielle Baeyens-Volant
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  7. Yvan Looze
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Brygier, J., Vlncentelli, J., Nljs, M. et al. Preparation and preliminary characterization of poly(ethylene glycol)-pepstatin conjugate. Appl Biochem Biotechnol 47, 1–10 (1994). https://doi.org/10.1007/BF02788670

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  • DOI: https://doi.org/10.1007/BF02788670

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