Abstract
Cellular fluorescence in vitro has been studied employing a low light-level video system interfaced with a real-time image array-processing computer system. Changes in cytoplasmic (mitochondrial) fluorescence in myocytes employing the probe rhodamine 6G have been studied over real time with the aid of several computer-based programs. An oscillating pattern of fluorescence is observed that appears to reflect localized variations in mitochondrial activity. The low light level video computer system used in this study compares favorably with laser-stimulated microspot fluorescence photon-counting techniques for the detection of subcellular fluorescence.
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References
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Berns, M.W., Siemens, A.E. & Walter, R.J. Mitochondrial fluorescence patterns in rhodamine 6G-stained myocardial cells in vitro. Cell Biophysics 6, 263–277 (1984). https://doi.org/10.1007/BF02788632
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DOI: https://doi.org/10.1007/BF02788632
Index Entries
- Mitochondrial fluorescence patterns
- fluorescence patterns, of mitochondrial
- rhodamine 6G, mitochondrial fluorescence patterns in
- myocardial cells, fluorescence patterns in
- computer video microscopy, of myocardial cells
- video microscopy, of myocardial cells
- microscopy, of mycardial cells by computer video
- laser microspot excitation, of myocardial cells
- excitation, of myocardial cells by laser microspot