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Separation and subfractionation of small numbers of cells (∼106) by countercurrent distribution in dextran-poly(ethylene glycol) aqueous-phase systems

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Abstract

Separation and subfractionation of cells on the basis of subtle differences in surface properties by partitioning in dextran-poly(ethylene glycol) aqueous phase systems is an established method. We report here that the incorporation of fetal bovine serum into such systems permits countercurrent distribution of small quantities of cells (∼106). In the absence of serum such small quantities of cells are lost (probably by adherence) and cannot be recovered after countercurrent distribution.

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Authors and Affiliations

  1. Laboratory of Chemical Biology, Veterans Administration Medical Center, 90822, Long Beach, CA

    Harry Walter & Eugene J. Krob

  2. Department of Physiology and Biophysics, University of California, 92717, Irvine, CA

    Harry Walter & Eugene J. Krob

Authors
  1. Harry Walter
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  2. Eugene J. Krob
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Walter, H., Krob, E.J. Separation and subfractionation of small numbers of cells (∼106) by countercurrent distribution in dextran-poly(ethylene glycol) aqueous-phase systems. Cell Biophysics 6, 253–262 (1984). https://doi.org/10.1007/BF02788631

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