Abstract
The rate of DNA synthesis along S-phase can be estimated on the basis of a simple model of a cell population, from a single FCM histogram under conditions of balanced exponential growth, and from two histograms suitably spaced in time under conditions of locally exponential S-phase influx. An algorithm that constructs a piecewise constant approximation of the synthesis rate and estimates the phase fractions from a single histogram is presented. The results of the application of the algorithm to a set of computer generated histograms in balanced exponential growth are reported.
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Bertuzzi, A., Gandolfi, A., Germani, A. et al. Estimation of cell DNA synthesis rate from flow-cytometric histograms. Cell Biophysics 5, 223–236 (1983). https://doi.org/10.1007/BF02788622
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DOI: https://doi.org/10.1007/BF02788622