Abstract
A recently proposed automatic procedure for analyzing DNA distribution from flow cytometric data is extensively tested against simulated data.
After a discussion of the procedure itself and of the simulation program, the results obtained are reported. They are evidence of the reliability of the procedure in extracting the proper underlying DNA distribution from sets of data obtained under various simulated instances. The different sources of error are then analyzed, along with their quantitative effects on the fit of the fluorescence histogram.
Similar content being viewed by others
References
Bruni, C., Koch, G., and Rossi, C. (1983),Cell Biophys. 5, 1.
Bruni, C., and Koch, G. (1982), Identifiability of continuous mixtures of unknown gaussian distributions. Report of the Instituto Automatica R 82-13, and Instituto Matematico “G. Castelnuovo,” University of Rome, Dec. 1982.Ann. Prob. to appear.
Fried, J., Perez, A. G., and Clarkson, B. D. (1976),J. Cell Biol. 71, 172.
Starace, G., private communication.
Bruni, C., Curzi, L., Koch, G., and Rossi, C. (1981), Biological aspects and mathematical analysis of the inverse problem in flow cytometry. Rept. IASI R 16, July 1981.
Baisch, H., Beck, H. P., Christensen, I. J., Hartmann, N. R., Fried, J., Dean, P. N., Gray, J. W., Jett, J. H., Johnston, D. A., White, R. A., Nicolini, C., Zeitz, S., and Watson, J. V. (1982),Cell Tissue Kinet. 15, 235.
Baisch, H., and Beck, H. P. (1978), Comparison of Cell Kinetic Parameters Obtained by Flow Cytometry and Autoradiography, inBiomathematics and Cell Kinetics (Valleron A. J., and Macdonald P. D. M., eds.), Elsevier/North Holland, Amsterdam, pp. 411–430.
Johnston, D. A., White, R. A., and Barlogie, B. (1978),Comp. Biomed. Res. 11, 393.
Van der Linden, P. M., (1980), Computer analysis of DNA histograms. Radiotherapy Department, Univ. of Utrecht.
Nedelman, J. (1982),Cell Biophys. 4, 1.
Del Bino, G., Bruni, C., Koch, G., Mazzini, G., Costa, A., and Silvestrini, R., (1982), A New Model for the Computer Analysis of Flow Cytometric DNA Data in Human Tumors, 6th International Symposium on Cytometry, Schloss Elmau, Oct., 1982.
Lucidi, S. (1982), La ricostruzione della distribuzione delle cellule rispetto al contenuto di DNA a partire da misure citofluorometriche. Un programma di simulazione per la generazione di distribuzioni citofluorometriche. Dissertation Abstract, February 1982.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bruni, C., Koch, G. & Lucidi, S. Analysis of DNA distributions from flow cytometry. Cell Biophysics 6, 67–86 (1984). https://doi.org/10.1007/BF02788588
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02788588