Applied Biochemistry and Biotechnology

, Volume 49, Issue 2, pp 153–164

Peroxidase-catalyzed polymerization and depolymerization of coal in organic solvents

  • Alexander M. Blinkovsky
  • James P. McEldoon
  • John M. Arnold
  • Jonathan S. Dordick
Article

DOI: 10.1007/BF02788549

Cite this article as:
Blinkovsky, A.M., McEldoon, J.P., Arnold, J.M. et al. Appl Biochem Biotechnol (1994) 49: 153. doi:10.1007/BF02788549

Abstract

Peroxidases from horseradish roots (HRP) and soybean hulls (SBP) catalyze the efficient polymerization of a 4-kDa dimethylformamide (DMF)-soluble fraction of Mequininza (Spanish) lignite in 50% (v/v) DMF with an aqueous component consisting of acetate buffer, pH 5.0. Under these conditions, HRP and SBP catalyze the oxidation of free phenolic moieties in the coal matrix, thereby leading to oxidative polymerization of the low-molecular-weight coal polymers. The high fraction of nonphenolic aromatic moieties in coal inspired us to examine conditions whereby such coal components could also become oxidized. Oxidation of nonphenolic aromatic compounds was attempted using veratryl alcohol as a model substrate. SBP catalyzed the facile oxidation of veratryl alcohol at pH <3.HRP, however, was unable to elicit veratryl alcohol oxidation. The potential for SBP to catalyze interunit bond cleavage on complex polymeric substrates was examined using l-(3,4-dimethoxyphenyl)-2-(phenoxy)propan-1,3-diol (1) as a substrate. SBP catalyzed the Cα-Cβ and β-ether bond cleavage of this compound, suggesting that similar reactions on coal, itself, could lead to depolymerization. Depolymerization of a >50 Da coal fraction was achieved using SBP in 50% (v/v) DMF with an aqueous component adjusted to pH 2.2. Approximately 15% of the initial high-molecular-weight lignite fraction was depolymerized to polymers 4 Da in size. Hence, SBP is capable of catalyzing the depolymerization of coal in organic solvents, and this may have important ramifications in the generation of liquid fuels from coals.

Index entries

Peroxidase from soybean hulls coal depolymerization enzymatic oxidation of veratryl alcohol 

Copyright information

© Human Press Inc 1994

Authors and Affiliations

  • Alexander M. Blinkovsky
    • 1
  • James P. McEldoon
    • 1
  • John M. Arnold
    • 1
  • Jonathan S. Dordick
    • 1
  1. 1.Department of Chemical and Biochemical Engineering and Center for Biocatalysis and BioprocessingUniversity of IowaIowa City

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