Summary
The effect of short-and long-term ethanol intake on digestive enzyme secretion was determined in isolated pancreatic lobules of rats. Groups of male Wistar rats were fed a modified Lieber-DeCarli diet containing either 5% (w/v) óf ethanol, isocaloric amounts of a liquid diet in which ethanol was substituted by starch, or solid rat chow; for 3 days, 1, 2, 4, 8 and 12 weeks. Basal and caerulein-stimulated secretion of lipase, amylase, chymotrypsin and trypsin and the enzyme content in the tissue were studied. Feeding the liquid control diet decreased the tissue content of the four enzymes as compared with the values obtained in the group receiving solid rat chow. While basal and stimulated amylase secretion was markedly reduced in the former group, the secretion pattern of the other enzymes exhibited only transient changes. Caerulein-stimulated secretion of lipase and the proteases was increased by ethanol, the effect being more pronounced during the initial phase of the experiment. Alcohol feeding stimulated the basal secretion of these enzymes only in weeks 1–4. In contrast to the other enzymes, basal and stimulated amylase secretion was not enhanced by ethanol feeding. The results suggest that the enzyme secretion of the rat pancreas is distinctly altered by chronic ethanol feeding. However, the response of the pancreatic enzymes is non-parallel, and changes with the duration of alcohol intake.
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References
Bode, J.C. Alcohol and the digestive system. In: D.P. Jewell and P.R. Gibson (Eds.), Topics in Gastroenterology, Vol. 12, Blackwell, Oxford, 1984: 91–98.
Singer, M.V. and Goebell, H. Acute and chronic actions of alcohol on pancreatic exocrine secretions in humans and animals, In: H.K. Seitz and B. Kommerell, (Eds.), Alcohol Related Diseases in Gastroenterology, Springer, Berlin, 1985: 376–414.
Singh, M., Effect of chronic ethanol feeding on pancreatic enzyme secretion in rats in vitro, Dig. Dis. Sci., 1983; 28: 117–123.
Sarles, H., Lebreul, G., Tasse, F., Figarella, C., Clemente, F., Devaux, M.A., Fagonde, B. and Payan, H., A comparison of alcoholic pancreatitis in rat and man, Gut, 1971; 12: 377–388.
Reber, H.A., Johnson, F.E., Montgomery, C.D., Carl W., and Wong, N.F., The effect of chronic alcohol ingestion on rat pancreas, Gastroenterology, 1976; 72: 94–117.
Kagaya, T., Takebe, T., Koizumi, M., Kataoka, S., Kamai, T. and Oyama K., Effect of long-term alcohol feeding on the pancreas in rat, Gastroenterol. Jpn., 1979; 14: 428–435.
Laugier, R. and Sarles, H., Effets de la consommation chronique d'alcool sur la sécrétion pancréatique exocrine du rat. Variations en fonction de la durée de consommation, Gastroenterol. Clin. Biol., 1977; 1: 767–774.
Jalovaara, P., Pancreatic exocrine secretion in the rat after chronic alcohol ingestion: nonparallel secretion of proteins and pancreatic secretory trypsin inhibitor, Scand. J. Gastroenterol., 1979; 14: 57–63.
Chariot, J., Rozé, C., de la Tour, J., Souchard, M., Hollande, E., Vaille, C. and Debray, C. Effects of chronic ethanol consumption on pancreatic response to central vagal stimulation by 2-deoxy-d-glucose in the rat, Digestion, 1977; 15: 425–437.
Singh, M., LaSure, M.M. and Bockman, D.E. Pancreatic acinar cell function and morphology in rats chronically fed an ethanol diet, Gastroenterology, 1982; 82: 425–434.
Scheele, G.P. and Palade, G.E. Studies on the guinea pig pancreas. Parallel discharge of exocrine enzyme activities, J. Biol. Chem., 1975; 250: 2660–2670.
DeCarli, L.M. and Lieber, C.S. Fatty liver in the rat after prolonged intake of ethanol with a nutritionally adequate new liquid diet, J. Nutr., 1967; 91: 331–336.
Rick, W. and Stegbauer, H. α-Amylase. Messung der reduzierenden Gruppen. In: H.U. Bergmeyer, (Ed.), Methoden der Enzymatischen Analyse, Vol. 1, Verlag Chemie, Weinheim, 1974: 919–923.
Näher, G. Lipase. Titrimetrische Messung, In: H.U. Bergmeyer (Ed.), Methoden der Enzymatischen Analyse, Vol. 1, Verlag Chemie, Weinheim, 1974: 843–848.
Hummel, B.C.W. A modified spectrophotometric determination of chymotrypsin, trypsin and thrombin, Can. J. Biochem. Physiol., 1959; 37: 1393–1399.
Richards, G.M. Modifications of the diphenylamine reaction giving increased sensitivity and simplicity in the estimation of DNA, Anal. Biochem., 1974; 57: 369–376.
Sarles, H. and Laugier, R. Alcoholic pancreatitis, Clinics Gastroenterol., 1981; 10: 401–415.
Tiscornia, O.M., Palasciano, G. and Sarles, H. Atropine and exocrine pancreatic secretion in alcoholic fed dogs, Am. J. Gastroenterol, 1975; 63: 33–36.
Demol, P., Andersen, B.N. and Sarles, H. Chronic ethanol consumption and exocrine pancreatic response to ethanol and acetaldehyde in the rat, Digestion, 1980; 20: 85–95.
Chariot, J., Le Bourhis, B. and Rozé, C. Response of rat exocrine pancreas to chronic pulmonary ethanol intoxication, Dig. Dis. Sci., 1980; 25: 656–659.
Uhlemann, E.R., Robberecht, P. and Gardner, J.D. Effects of alcohol on the actions of VIP and secretin on acinar cells from guinea pig pancreas. Gastroenterology, 1979; 76: 917–925.
Sarles, H., Figarella, C. and Clemente, F. The interaction of ethanol, dietary lipids, and proteins on the rat pancreas, Digestion, 1971; 4: 12–22.
Bayer, M., Rudick, J., Lieber, C.S. and Janowitz, H.D. Inhibitory effects of ethanol on canine exocrine pancreatic secretion, Gastroenterology, 1972; 63: 619–626.
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Bode, C., Dürr, H.K. & Bode, J.C. Effect of short- and long-term alcohol feeding in rats on pancreatic enzyme content and enzyme secretion in isolated pancreatic lobules in vitro. Int J Pancreatol 1, 129–139 (1986). https://doi.org/10.1007/BF02788445
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DOI: https://doi.org/10.1007/BF02788445