Abstract
The cultures of chicken embryo fibroblast (CEF) cells in flasks, spinner bottles, and bioreactors were studied. The growth and metabolism characteristics of CEF cells and the feasibility of the CEF cell culture in bioreactor were investigated. The plating process of the CEF cells on GT-2 microcarriers in spinner bottles was studied, and a plating kinetic model was presented. The culture of CEF cells in 1.5 L CelliGen bioreactor to produce infectious bursal disease virus (IBDV) had met success. Whereas the additive microcarriers were fed during the culture, the cell density was increased 10 times as against seed cells adhering to microcarriers and the virus titer was as high as 7.5. All the aforementioned experimental results have laid the foundation for high density culture of CEF cells and process scale-up.
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Abbreviations
- Μ:
-
specific cell growth rate (d-1)
- qglu:
-
specific glucose consumption rate (mg/105 cells/day)
- qLac:
-
specific lactate accumulation rate (mg/105 cells/day)
- qNH4+:
-
specific ammonia accumulation rate (mg/105 cells/day)
- CT:
-
total inoculum cell density (cells/mL)
- a:
-
cell viability
- CMC:
-
microcarrier concentration (mg/mL)
- CS:
-
cells having plated on microcarriers tightly (cells/mL)
- K:
-
the equilibrium constant
- k2 :
-
the rate constant
- Φ:
-
plating ratio
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Zhang, L., Zhang, Y., Yan, C. et al. The culture of chicken embryo fibroblast cells on microcarriers to produce infectious bursal disease virus. Appl Biochem Biotechnol 62, 291–302 (1997). https://doi.org/10.1007/BF02788004
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DOI: https://doi.org/10.1007/BF02788004