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Continuous production of halophilic α- amylase through whole cell immobilization ofhalobacterium salinarium

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Abstract

The cells ofHalobacterium salinarium were immobilized in calcium alginate beads and a polyvinyl alcohol film with an aim to improve the production of halophilicα-amylase. The cells ofHalobacterium salinarium were stabilized by cross-linking with 0.5% glutaraldehyde. Such cells were found to be osmotically stable and showed continuous production of the enzyme for several days (45 d). The stabilized cells could be permeabilized by treatment with chloroform without leakage of intracellular components. This technique can serve as a tool for studyingin situ regulatory characteristics of intracellular functions in halobacteria and can also help in their reuse under more stabilized conditions for biotechnological applications.

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Authors and Affiliations

  1. Post-Graduate Department of Biosciences, Sardar Patel University,Vallabh Vidyanagar, 388 120, Gujarat, India

    Rajvit Bagai & Datta Madamwar

Authors
  1. Rajvit Bagai
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  2. Datta Madamwar
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Bagai, R., Madamwar, D. Continuous production of halophilic α- amylase through whole cell immobilization ofhalobacterium salinarium . Appl Biochem Biotechnol 62, 213–218 (1997). https://doi.org/10.1007/BF02787997

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  • DOI: https://doi.org/10.1007/BF02787997

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