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Novel functional activities of anti-dna autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases

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Abstract

DNA-hydrolyzing activity of IgG autoantibodies from sera of patients with various types of lymphoproliferative diseases was investigated. The association of DNA-hydrolyzing activity with the antibody (Ab) fraction has been proved by newly developed affinity-capture assay. Study of abzyme incidence in blood tumors and systemic lupus erythematosis (SLE) revealed linkage of anti-DNA Ab catalysts to mature B-cell tumors, and increased probability of DNA-abzymes formation on the background of autoimmune manifestations. These data suggest possible similarity between mechanisms of abzyme formation in SLE and B-cell lymphomas. A new mechanism of formation of DNA-specific catalytic Abs has been proposed based on the increased crossreactivity of polyclonal DNA-abzymes to DNA-depleted nuclear matrix proteins. The possibility of the abzyme production as Ab to the energetically destabilized ground state of the antigen has been discussed. Preliminary results were obtained that indicate the complement-independent cytotoxicity of anti-DNA autoantibodies isolated from blood of patients with SLE and chronic lymphocytic leukemia.

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References

  1. Paul, S., Volle, D.J., Beach, C. M., Johnson, D. R., Powell, M. J., and Massey, R. J. (1989),Science 244, 1158–1161.

    Article  CAS  Google Scholar 

  2. Li, L., Paul, S., Tyutyulkova, S., Kazatchkine, M. D., and Kaveri, S. (1995),J. Immunol. 154, 3328–3332.

    CAS  Google Scholar 

  3. Schuster, A. M., Gololobov, G. V., Kvashuk, O. A., Bogomolova, A. E., Smirnov, I. V., and Gabibov, A. G. (1992),Science 256, 665–667.

    Article  Google Scholar 

  4. Gololobov, G. V., Mikhalap, S. V., Starov, A. V., Kolesnikov, A. V., and Gabibov, A. G. (1994),App. Biochem. Biotechnol. 47, 305–317.

    CAS  Google Scholar 

  5. Naparstek, Y. and Plotz, P. H. (1993),Ann. Rev. Immunol. 11, 79–104.

    Article  CAS  Google Scholar 

  6. Suzuki, N., Mihara, S., and Sakane, T. (1997),FASEB J. 11, 1033–1038.

    CAS  Google Scholar 

  7. Dighiero, G. (1992),Leukemia and Lymphoma 8, 345–351.

    Article  CAS  Google Scholar 

  8. Shoenfeld, Y., Amitol-Teplizki, H., Mendlovic, S., Blank, M., Mozec, E., and Isenberg, D. A. (1989),Clin. Immunol. Immunopathol. 51, 313–325.

    Article  CAS  Google Scholar 

  9. Paul, S., Li, L., Kalaga, R., Wilkins-Stevens, P., Stevens, F. J., and Solomon, A. (1995),J. Biol. Chem. 270, 15,257–15,261.

    Article  CAS  Google Scholar 

  10. Kozyr, A. V. (1996),Biochem. Mol. Biol. Int. 39, 403–413.

    CAS  Google Scholar 

  11. Schuster, A. M., Gololobov, G. V., Kvashuk, O. A., and Gabibov, A. G. (1991),Mol. Biol. (Russian)25, 593–602.

    Google Scholar 

  12. Kohwi-Shigematsu, T., deBelle, I., Dickinson, L. A., Galande, S., and Kohwi, Y. (1998),Methods Cell Biol. 53, 323–354.

    Article  CAS  Google Scholar 

  13. Alarcon-Segovia, D., Ruiz-Arguelles, A., and Llorente, L. (1996),Immunol. Today 17, 163–164.

    Article  CAS  Google Scholar 

  14. Ras, E., Ben-Bassat, H., Davidi, T., Shlomai, Z., and Eilat, D. (1993),Eur. J. Immunol. 23, 383–390.

    Article  Google Scholar 

  15. Yanase, K., Smith, R. M., Puccetti, A., Jarett, L., and Madaio, M. P. (1997),J. Clin. Invest. 100, 25–31.

    CAS  Google Scholar 

  16. Stemmer, C., Richalet-Secordel, P., van Bruggen, M., Kramers, K., Berden, J., and Muller, S. (1996),J. Biol. Chem. 271, 21,257–21,261.

    Article  CAS  Google Scholar 

  17. Sashchenko, L. P., Gnuchev, N. V., Kirillova, M. A., Lukjanova, T. I., Rebizova, T. V., and Lukanidin, E. M. (1988),FEBS Lett. 266, 261–264.

    Article  Google Scholar 

  18. Belgrader, P., Siegel, A. J., and Berezney, R. (1991),J. Cell. Sci. 98, 289–291.

    Google Scholar 

  19. Sambrook, J., Fritsch, E. E., and Maniatis, T. (1989),Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, part 5.43.

    Google Scholar 

  20. Gabibov, A. G., Gololobov, G. V., Makarevich, O. I., Schourov, D. V., Chernova, E. A., and Yadav, R. P. (1994),Appl. Biochem. Biotechnol. 47, 293–302.

    CAS  Google Scholar 

  21. Sthoeger, Z. M., Wakai, M., Tse, D. B., Vinciguerra, V. P., Allen, S. L., Budman, D. R., et al. (1989),J. Exp. Med. 169, 255–268.

    Article  CAS  Google Scholar 

  22. Paul, W. E., ed. (1987),Fundamental Immunology, Raven, New York (Russian edition: Mir, Moscow p. 209.)

    Google Scholar 

  23. Zack, D. J., Stempniak, M., Wong, A. L., Taylor, C., Weisbart, R. H. (1996),J. Immunol. 157, 2082–2088.

    CAS  Google Scholar 

  24. Engelmann, H., Holtmann, H., Brakebusch, C., Avni, Y. S., Sarov, L., Nophar, Y., (1990),J. Biol. Chem. 265, 14,497–14,504.

    CAS  Google Scholar 

  25. Mirkina, I. I., Mernenko, O. A., Satpaev, D. K., Karelin, A. A., and Blishenko, E. Yu. (1996),Immunol. Lett. 52, 105–108.

    Article  CAS  Google Scholar 

  26. Bronshtein, I. B., Shuster, A. M., Gololobov, G. V., Gromova, I. I., Kvashuk, O. A., Belostotskaya, K. M., et al. (1992),FEBS Lett. 314, 259–263.

    Article  CAS  Google Scholar 

  27. Van Regenmortel, M. H. V. (1995),Biomed. Pept. Protein Nucleic Acids 1, 109–116.

    Google Scholar 

  28. Aleksandrova, E. S. (1996),Mol. Biol. (Russian),30, 921–926.

    CAS  Google Scholar 

  29. Gololobov, G. V., Rumbley, K., Rumbley, J., Schourov, D. V., Makarevich, O. I., Gabibov, A. G., Voss, E., and Rodkey, S. (1997),Mol. Immunol. 34, 1083–1093.

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Shemyokin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St., 16/10, 117871, V-437, Moscow, Russia

    A. V. Kozyr, A. V. Kolesnikov & A. G. Gabibov

  2. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia

    E. S. Aleksandrova, P. V. Favorov, M. A. Kotelnikov & A. G. Gabibov

  3. Institute of Gene Biology, Russian Academy of Sciences, Moscow, Russia

    L. P. Sashchenko & N. V. Gnuchev

  4. Russian Hematological Center, Russian Academy of Medical Sciences, Moscow, Russia

    E. I. Iakhnina & I. A. Astsaturov

  5. Institute of Rheumatology, Russian Academy of Medical Sciences, Moscow, Russia

    T. B. Prokaeva & Z. S. Alekberova

  6. Moscow Regional Research Institute of Clinical Investigations, Moscow, Russia

    S. V. Suchkov

Authors
  1. A. V. Kozyr
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  2. A. V. Kolesnikov
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  3. E. S. Aleksandrova
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  4. L. P. Sashchenko
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  5. N. V. Gnuchev
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  6. P. V. Favorov
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  7. M. A. Kotelnikov
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  8. E. I. Iakhnina
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  9. I. A. Astsaturov
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  10. T. B. Prokaeva
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  11. Z. S. Alekberova
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  12. S. V. Suchkov
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  13. A. G. Gabibov
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Corresponding author

Correspondence to A. G. Gabibov.

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Kozyr, A.V., Kolesnikov, A.V., Aleksandrova, E.S. et al. Novel functional activities of anti-dna autoantibodies from sera of patients with lymphoproliferative and autoimmune diseases. Appl Biochem Biotechnol 75, 45–61 (1998). https://doi.org/10.1007/BF02787708

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  • DOI: https://doi.org/10.1007/BF02787708

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